Promoter diversity in multigene transformation

被引：109

作者：

Peremarti, Ariadna ^{[2
]}

Twyman, Richard M. ^{[3
]}

Gomez-Galera, Sonia ^{[2
]}

Naqvi, Shaista ^{[2
]}

Farre, Gemma ^{[2
]}

Sabalza, Maite ^{[2
]}

Miralpeix, Bruna ^{[2
]}

Dashevskaya, Svetlana ^{[2
]}

Yuan, Dawei ^{[2
]}

Ramessar, Koreen ^{[2
]}

Christou, Paul ^{[1
,2
]}

Zhu, Changfu ^{[2
]}

Bassie, Ludovic ^{[2
]}

Capell, Teresa ^{[2
]}

机构：

[1] Inst Catalana Recerca & Estudis Avancats, Bellaterra, Spain

[2] Univ Lleida, ETSEA, Dept Prod Vegetal & Ciencia Forestal, Lleida 25198, Spain

[3] Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England

来源：

PLANT MOLECULAR BIOLOGY | 2010年 / 73卷 / 4-5期

基金：

欧洲研究理事会;

关键词：

Promoter; Transgene; Multigene transformation; Transcriptional silencing; Constitutive; Spatiotemporal; Inducible; HIGH-LEVEL EXPRESSION; ENDOSPERM-SPECIFIC EXPRESSION; INTRON-MEDIATED ENHANCEMENT; TISSUE-SPECIFIC EXPRESSION; CIS-REGULATORY ELEMENTS; MOTTLE VIRUS PROMOTER; BRASSICA-NAPUS SEEDS; LEAF-CURLING-VIRUS; RNA-POLYMERASES-IV; 5' FLANKING REGION;

D O I：

10.1007/s11103-010-9628-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Multigene transformation (MGT) is becoming routine in plant biotechnology as researchers seek to generate more complex and ambitious phenotypes in transgenic plants. Every nuclear transgene requires its own promoter, so when coordinated expression is required, the introduction of multiple genes leads inevitably to two opposing strategies: different promoters may be used for each transgene, or the same promoter may be used over and over again. In the former case, there may be a shortage of different promoters with matching activities, but repetitious promoter use may in some cases have a negative impact on transgene stability and expression. Using illustrative case studies, we discuss promoter deployment strategies in transgenic plants that increase the likelihood of successful and stable multiple transgene expression.

引用

页码：363 / 378

页数：16

共 158 条

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