CTG triplet repeats from human hereditary diseases are dominant genetic expansion products in Escherichia coli

被引：43

作者：

Ohshima, K ^{[1
]}

Kang, S ^{[1
]}

Wells, RD ^{[1
]}

机构：

[1] TEXAS A&M UNIV, TEXAS MED CTR, CTR GENOME RES, INST BIOSCI & TECHNOL, HOUSTON, TX 77030 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1996年 / 271卷 / 04期

关键词：

D O I：

10.1074/jbc.271.4.1853

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The relative ability of the 10 triplet repeat sequences to be expanded in Escherichia coli was determined. Surprisingly, CTG; tracts are expanded at least 8 times more frequently than any of the other nine triplets. Low levels of expansion were found also for CGG;, GTG, and GTC. Thus, the structure of the CTG repeats and/or their utilization by the DNA synthetic systems in vivo must be quite different from the other triplets. These data further validate this genetically defined system for elucidating molecular mechanisms of expansion and may explain why most triplet repeat hereditary neuromuscular and neurodegenerative disease genes contain CTG repeats.

引用

页码：1853 / 1856

页数：4

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