Efficient production of neural stem cells and neurons from embryonic stem cells

被引：49

作者：

Nakayama, T

Momoki-Soga, T

Yamaguchi, K

Inoue, N ^{[1
]}

机构：

[1] Yokohama City Univ, Sch Med, Dept Biochem 1, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan

[2] RIKEN, Brain Sci Inst, Lab Memory & Learning, Wako, Saitama 3510198, Japan

来源：

NEUROREPORT | 2004年 / 15卷 / 03期

关键词：

astrocyte-conditioned medium; dopaminergic neurons; ES cells; neural stem cells; neural stem spheres; neuronal differentiation;

D O I：

10.1097/00001756-200403010-00021

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have developed a simple method to efficiently produce a large number of neural stem cells and neurons from mouse embryonic stem (ES) cells. When cultured in astrocyte-conditioned medium (ACM) with mitogens (FGF-2 and EGF) under free-floating conditions, colonies of undifferentiated ES cells give rise to neural stem spheres (NSSs), composed of plentiful neural stem cells. Subsequent culture of the NSSs on an adhesive substrate with mitogens results in the migration of neural stem cells onto the substrate. These cells can be expanded, preserved by freezing, and differentiated into functional neurons. Neural stem cells and neurons provided by this NSS method may be valuable as potential donor cells for neuronal transplantation and also as convenient alternatives to tissue-derived neural cells.

引用

收藏

页码：487 / 491

页数：5

相关论文

共 22 条

[1] EMBRYONIC STEM-CELLS EXPRESS NEURONAL PROPERTIES IN-VITRO [J].

BAIN, G ;

KITCHENS, D ;

YAO, M ;

HUETTNER, JE ;

GOTTLIEB, DI .

DEVELOPMENTAL BIOLOGY, 1995, 168 (02) :342-357

[2] BRAIN BASIC FIBROBLAST GROWTH-FACTOR STIMULATES THE PROLIFERATION OF RAT NEURONAL PRECURSOR CELLS-INVITRO [J].

GENSBURGER, C ;

LABOURDETTE, G ;

SENSENBRENNER, M .

FEBS LETTERS, 1987, 217 (01) :1-5

[3] Basis and clinical neuroscience applications of embryonic stem cells [J].

Gökhan, S ;

Mehler, MF .

ANATOMICAL RECORD, 2001, 265 (03) :142-156

[4] Embryonic stem cell-derived neurogenesis - Retinoic acid induction and lineage selection of neuronal cells [J].

Guan, KM ;

Chang, H ;

Rolletschek, A ;

Wobus, AM .

CELL AND TISSUE RESEARCH, 2001, 305 (02) :171-176

[5] IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES [J].

HAMILL, OP ;

MARTY, A ;

NEHER, E ;

SAKMANN, B ;

SIGWORTH, FJ .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1981, 391 (02) :85-100

[6] THE APPEARANCE OF A HIGHLY DIGITALIS-SENSITIVE ISOFORM OF NA+,K+-ATPASE DURING MATURATION INVITRO OF PRIMARY CULTURED RAT CEREBRAL NEURONS [J].

INOUE, N ;

MATSUI, H ;

TSUKUI, H ;

HATANAKA, H .

JOURNAL OF BIOCHEMISTRY, 1988, 104 (03) :349-354

[7] Generation of dopaminergic neurons and pigmented epithelia from primate ES cells by stromal cell-derived inducing activity [J].

Kawasaki, H ;

Suernori, H ;

Mizuseki, K ;

Watanabe, K ;

Urano, F ;

Ichinose, H ;

Haruta, M ;

Takahashi, M ;

Yoshikawa, K ;

Nishikawa, SI ;

Nakatsuji, N ;

Sasai, Y .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (03) :1580-1585

[8] Induction of midbrain dopaminergic neurons from ES cells by stromal cell-derived inducing activity [J].

Kawasaki, H ;

Mizuseki, K ;

Nishikawa, S ;

Kaneko, S ;

Kuwana, Y ;

Nakanishi, S ;

Nishikawa, S ;

Sasai, Y .

NEURON, 2000, 28 (01) :31-40

[9] Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cells [J].

Lee, SH ;

Lumelsky, N ;

Studer, L ;

Auerbach, JM ;

McKay, RD .

NATURE BIOTECHNOLOGY, 2000, 18 (06) :675-679

[10] CNS STEM-CELLS EXPRESS A NEW CLASS OF INTERMEDIATE FILAMENT PROTEIN [J].

LENDAHL, U ;

ZIMMERMAN, LB ;

MCKAY, RDG .

CELL, 1990, 60 (04) :585-595