Chronic hepatitis C viral infection reduces NK cell frequency and suppresses cytokine secretion: Reversion by anti-viral treatment

被引：57

作者：

Dessouki, Omar ^{[1
]}

Kamiya, Yasuyuki ^{[2
]}

Nagahama, Hiroyasu ^{[2
]}

Tanaka, Motohiko ^{[2
]}

Suzu, Shinya ^{[1
]}

Sasaki, Yutaka ^{[2
]}

Okada, Seiji ^{[1
]}

机构：

[1] Kumamoto Univ, Div Hematopoiesis, Ctr AIDS Res, Kumamoto 8600811, Japan

[2] Kumamoto Univ, Dept Gastroenterol & Hepatol, Grad Sch Med Sci, Kumamoto 8600811, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 393卷 / 02期

关键词：

Hepatitis C; Natural killer cells; Subsets; IFN-gamma; CD107a; NATURAL-KILLER-CELLS; INTERFERON-ALPHA; VIRUS; CYTOTOXICITY; ACTIVATION; EXPRESSION; RECEPTORS; RIBAVIRIN; RESPONSES; CD81;

D O I：

10.1016/j.bbrc.2010.02.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

impaired activity of NK (natural killer) cells has been proposed as a mechanism contributing to viral persistence and chronic infection in hepatitis C (HCV) infection. We aimed to assess the impact of HCV infection on NK cells regarding frequency, subset distribution, and cytotoxic and cytokine secretion functions, as well as IFN-alpha and ribavirin therapeutic effects on NK cells. Significant reduction of total NK frequency and the CD56(dim)16(+) subset was observed in chronic HCV patients. IFN-gamma expression upon stimulation with K562 was severely suppressed but cytotoxicity measured by CD107a expression was maintained. These adverse effects were reversed after treatment with pegylated IFN-alpha and ribavirin; however, these skewed functions were not recovered in treatment-resistant patients. Thus, HCV chronic infection severely affects NK functions, except for cytotoxicity. Altered NK cell frequency and cytokine secretion by HCV infection may contribute to impaired cellular immune response and virus persistence. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：331 / 337

页数：7

共 26 条

[1] Natural Killer Cells Are Polarized Toward Cytotoxicity in Chronic Hepatitis C in an Interferon-Alfa-Dependent Manner [J].

Ahlenstiel, Golo ;

Titerence, Rachel H. ;

Koh, Christopher ;

Edlich, Birgit ;

Feld, Jordan J. ;

Rotman, Yaron ;

Ghany, Marc G. ;

Hoofnagle, Jay H. ;

Liang, T. Jake ;

Heller, Theo ;

Rehermann, Barbara .

GASTROENTEROLOGY, 2010, 138 (01) :325-335

[2] CD107a as a functional marker for the identification of natural killer cell activity [J].

Alter, G ;

Malenfant, JM ;

Altfeld, M .

JOURNAL OF IMMUNOLOGICAL METHODS, 2004, 294 (1-2) :15-22

[3] Activation and function of natural killer cell responses during viral infections [J].

Biron, CA .

CURRENT OPINION IN IMMUNOLOGY, 1997, 9 (01) :24-34

[4] Fine characterization of intrahepatic NK cells expressing natural killer receptors in chronic hepatitis B and C [J].

Bonorino, Paula ;

Ramzan, Muhammad ;

Camous, Xavier ;

Dufeu-Duchesne, Tania ;

Thelu, Marie-Ange ;

Sturm, Nathalie ;

Dariz, Aurelie ;

Guillermet, Christiane ;

Pernollet, Martine ;

Zarski, Jean-Pierre ;

Marche, Patrice N. ;

Leroy, Vincent ;

Jouvin-Marche, Evelyne .

JOURNAL OF HEPATOLOGY, 2009, 51 (03) :458-467

[5] Inhibition of natural killer cells through engagement of CD81 by the major hepatitis C virus envelope protein [J].

Crotta, S ;

Stilla, A ;

Wack, A ;

D'Andrea, A ;

Nuti, S ;

D'Oro, U ;

Mosca, M ;

Filliponi, F ;

Brunetto, RM ;

Bonino, F ;

Abrignani, S ;

Valiante, NM .

JOURNAL OF EXPERIMENTAL MEDICINE, 2002, 195 (01) :35-41

[6] Increased natural cytotoxicity receptor expression and relevant IL-10 production in NK cells from chronically infected viremic HCV patients [J].

De Maria, Andrea ;

Fogli, Manuela ;

Mazza, Stefania ;

Basso, Monica ;

Picciotto, Antonio ;

Costa, Paola ;

Congia, Sonia ;

Mingari, Maria Cristina ;

Moretta, Lorenzo .

EUROPEAN JOURNAL OF IMMUNOLOGY, 2007, 37 (02) :445-455

[7] Control of antiviral defenses through hepatitis C virus disruption of retinoic acid-inducible gene-I signaling [J].

Foy, E ;

Li, K ;

Sumpter, R ;

Loo, YM ;

Johnson, CL ;

Wang, CF ;

Fish, PM ;

Yoneyama, M ;

Fujita, T ;

Lemon, SM ;

Gale, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (08) :2986-2991

[8] Regulation of interferon regulatory factor-3 by the hepatitis C virus serine protease [J].

Foy, E ;

Li, K ;

Wang, CF ;

Sumpter, R ;

Ikeda, M ;

Lemon, SM ;

Gale, M .

SCIENCE, 2003, 300 (5622) :1145-1148

[9] Peginterferon alfa-2a plus ribavirin for chronic hepatitis C virus infection. [J].

Fried, MW ;

Shiffman, ML ;

Reddy, KR ;

Smith, C ;

Marinos, G ;

Goncales, FL ;

Haussinger, D ;

Diago, M ;

Carosi, G ;

Dhumeaux, D ;

Craxi, A ;

Lin, A ;

Hoffman, J ;

Yu, J .

NEW ENGLAND JOURNAL OF MEDICINE, 2002, 347 (13) :975-982

[10] Expansion of CD56- NK cells in chronic HCV/HIV-1 co-infection:: Reversion by antiviral treatment with pegylated IFNα and ribavirin [J].

Gonzalez, Veronica D. ;

Falconer, Karolin ;

Michaelsson, Jakob ;

Moll, Markus ;

Reichard, Olle ;

Alaeus, Annette ;

Sandberg, Johan K. .

CLINICAL IMMUNOLOGY, 2008, 128 (01) :46-56

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