Integrated genomic and proteomic analyses of gene expression in mammalian cells

被引:633
作者
Tian, Q
Stepaniants, SB
Mao, M
Weng, L
Feetham, MC
Doyle, MJ
Yi, EC
Dai, HY
Thorsson, V
Eng, J
Goodlett, D
Berger, JP
Gunter, B
Linseley, PS
Stoughton, RB
Aebersold, R
Collins, SJ
Hanlon, WA
Hood, LE
机构
[1] Inst Syst Biol, Seattle, WA 98103 USA
[2] Merck Res Labs, Rosetta Inpharmat, Seattle, WA 98109 USA
[3] Fred Hutchinson Canc Res Ctr, Human Biol Div, Seattle, WA 98125 USA
关键词
D O I
10.1074/mcp.M400055-MCP200
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Using DNA microarrays together with quantitative proteomic techniques (ICAT reagents, two-dimensional DIGE, and MS), we evaluated the correlation of mRNA and protein levels in two hematopoietic cell lines representing distinct stages of myeloid differentiation, as well as in the livers of mice treated for different periods of time with three different peroxisome proliferative activated receptor agonists. We observe that the differential expression of mRNA ( up or down) can capture at most 40% of the variation of protein expression. Although the overall pattern of protein expression is similar to that of mRNA expression, the incongruent expression between mRNAs and proteins emphasize the importance of posttranscriptional regulatory mechanisms in cellular development or perturbation that can be unveiled only through integrated analyses of both proteins and mRNAs.
引用
收藏
页码:960 / 969
页数:10
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