Neurotrophins increase motoneurons' ability to innervate skeletal muscle fibers in rat spinal cord-human muscle cocultures

被引：66

作者：

Braun, S

Croizat, B

Lagrange, MC

Warter, JM

Poindron, P

机构：

[1] UNIV LOUIS PASTEUR STRASBOURG 1,UFR SCI PHARMACEUT,DEPT IMMUNOL IMMUNOPHARMACOL & PATHOL,F-67401 ILLKIRCH GRAFFENS,FRANCE

[2] COLL FRANCE,F-75231 PARIS,FRANCE

[3] ASSOC FRANCAISE CONTRE MYOPATHIES,PARIS,FRANCE

来源：

JOURNAL OF THE NEUROLOGICAL SCIENCES | 1996年 / 136卷 / 1-2期

关键词：

neurotrophin; synaptogenesis; neurite outgrowth; in vitro; rat; human;

D O I：

10.1016/0022-510X(95)00315-S

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Neurotrophins, nerve growth factor (NGF), neurotrophin-3 (NT-3), neurotrophin-5 (NT-5) and brain-derived neurotrophic factor (BDNF), were studied in vitro in a coculture model of human skeletal muscle myotubes and rat embryo spinal cord explants, which enables the different steps of functional innervation to be followed, including neurite outgrowth, synapse formation and induction of contractile activity, We found that NT-3, NT-5, BDNF, but not NGF simultaneously induced a significant increase in the number and length of neurites emerging from spinal cord explants, the number of endplates per muscle fiber, and the area of innervated muscle fibers around each spinal cord explant. These results suggest that neurotrophins NT-3, NT-5 and BDNF enhance spinal cord motoneurons potential of innervation.

引用

页码：17 / 23

页数：7

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