Technical advancement in regulatory T cell isolation and characterization using CD127 expression in patients with malignant glioma treated with autologous dendritic cell vaccination

被引：18

作者：

Ardon, H. ^{[1
,2
]}

Verbinnen, B. ^{[2
]}

Maes, W. ^{[2
]}

Beez, T. ^{[2
,3
]}

Van Cool, S. ^{[2
,4
]}

De Vleeschouwer, S. ^{[1
,2
]}

机构：

[1] Univ Hosp Leuven, Dept Neurosurg, B-3000 Louvain, Belgium

[2] Univ Hosp Leuven, Expt Immunol Lab, B-3000 Louvain, Belgium

[3] Univ Hosp Heinrich Heine, Inst Transplantat Diagnost & Cell Therapeut, Dusseldorf, Germany

[4] Univ Hosp Leuven, Dept Paediat, B-3000 Louvain, Belgium

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2010年 / 352卷 / 1-2期

关键词：

Glioma; DC vaccination; Monitoring; Treg; Foxp3; CD127; FOXP3;

D O I：

10.1016/j.jim.2009.10.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have successfully treated over two hundred high-grade glioma (HGG) patients with immunotherapy consisting of vaccination with autologous dendritic cells (DCs) loaded with autologous tumour lysate. It has been documented that regulatory T cells (Treg) can counteract anti-tumour immune responses. Therefore, monitoring of Treg in these patients is essential. Up till now, Treg have been characterized based on the expression of the transcription factor Foxp3. Here, we validated IL-7 receptor alpha subunit (CD127)dim expression as a marker for human Treg within HGG patients, as a less laborious assay for routine use in tumour vaccination trials. We noted a strong positive correlation between Foxp3 expression and CD127dim expression in CD4 + CD25+ and CD4+ cells. The suppressive function of CD4 + CD127dim cells was assessed in an allogeneic mixed lymphocyte reaction (MLR). We conclude that CD127 staining is a fast, well-suited and reproducible Treg monitoring tool in HGG patients treated with immunotherapy. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：169 / 173

页数：5

共 13 条

[1] Postoperative adjuvant dendritic cell-based immunotherapy in patients with relapsed glioblastoma multiforme [J].

De Vleeschouwer, Steven ;

Fieuws, Steffen ;

Rutkowski, Stefan ;

Van Calenbergh, Frank ;

Van Loon, Johannes ;

Goffin, Jan ;

Sciot, Raf ;

Wilms, Guido ;

Demaerel, Philippe ;

Warmuth-Metz, Monika ;

Soerensen, Niels ;

Wolff, Johannes E. A. ;

Wagner, Sabine ;

Kaempgen, Eckhart ;

Van Gool, Stefaan W. .

CLINICAL CANCER RESEARCH, 2008, 14 (10) :3098-3104

[2]

Dunn Gavin P, 2007, Cancer Immun, V7, P12

[3] Increased regulatory T-cell fraction amidst a diminished CD4 compartment explains cellular immune defects in patients with malignant glioma. [J].

Fecci, PE ;

Mitchell, DA ;

Whitesides, JF ;

Xie, WH ;

Friedman, AH ;

Archer, GE ;

Herndon, JE ;

Bigner, DD ;

Dranoff, G ;

Sampson, JH .

CANCER RESEARCH, 2006, 66 (06) :3294-3302

[4]

Gomez GG, 2006, GENE THER MOL BIOL, V10A, P133

[5] Elimination of regulatory T cells is essential for an effective vaccination with tumor lysate-pulsed dendritic cells in a murine glioma model [J].

Grauer, Oliver M. ;

Sutmuller, Roger P. M. ;

van Maren, Wendy ;

Jacobs, Joannes F. M. ;

Bennink, Erik ;

Toonen, Liza W. J. ;

Nierkens, Stefan ;

Adema, Gosse J. .

INTERNATIONAL JOURNAL OF CANCER, 2008, 122 (08) :1794-1802

[6] CD4+FoxP3+regulatory T cells gradually accumulate in gliomas during tumor growth and efficiently suppress antiglioma immune responses in vivo [J].

Grauer, Oliver M. ;

Nierkens, Stefan ;

Bennink, Erik ;

Toonen, Liza W. J. ;

Boon, Louis ;

Wesseling, Pieter ;

Sutmuller, Roger P. M. ;

Adema, Gosse J. .

INTERNATIONAL JOURNAL OF CANCER, 2007, 121 (01) :95-105

[7] Incidence and prognostic impact of FoxP3+ regulatory T cells in human gliomas [J].

Heimberger, Amy B. ;

Abou-Ghazal, Mohamed ;

Reina-Ortiz, Chantal ;

Yang, David S. ;

Sun, Wei ;

Qiao, Wei ;

Hiraoka, Nobuyoshi ;

Fuller, Gregory N. .

CLINICAL CANCER RESEARCH, 2008, 14 (16) :5166-5172

[8] Regulatory T cells and the PD-L1/PD-1 pathway mediate immune suppression in malignant human brain tumors [J].

Jacobs, Joannes F. M. ;

Idema, Albert J. ;

Bol, Kalijn F. ;

Nierkens, Stefan ;

Grauer, Oliver M. ;

Wesseling, Pieter ;

Grotenhuis, J. Andre ;

Hoogerbrugge, Peter M. ;

de Vries, I. Jolanda M. ;

Adema, Gosse J. .

NEURO-ONCOLOGY, 2009, 11 (04) :394-402

[9] CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cells [J].

Liu, Weihong ;

Putnam, Amy L. ;

Xu-yu, Zhou ;

Szot, Gregory L. ;

Lee, Michael R. ;

Zhu, Shirley ;

Gottlieb, Peter A. ;

Kapranov, Philipp ;

Gingeras, Thomas R. ;

Fazekas de St Groth, Barbara ;

Clayberger, Carol ;

Soper, David M. ;

Ziegler, Steven F. ;

Bluestone, Jeffrey A. .

JOURNAL OF EXPERIMENTAL MEDICINE, 2006, 203 (07) :1701-1711

[10] DC vaccination with anti-CD25 treatment leads to long-term immunity against experimental glioma [J].

Maes, Wim ;

Rosas, Georgina Galicia ;

Verbinnen, Bert ;

Boon, Louis ;

De Vleeschouwer, Steven ;

Ceuppens, Jan L. ;

Van Gool, Stefaan W. .

NEURO-ONCOLOGY, 2009, 11 (05) :529-542

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