Comparing AFLP, RAPD and RFLP markers for measuring genetic diversity in melon

被引:148
作者
Garcia-Mas, J
Oliver, M
Gómez-Paniagua, H
de Vicente, MC
机构
[1] IRTA, Ctr Cabrils, Dept Genet Vegetal, Cabrils 08348, Spain
[2] Semillas Fito SA, Barcelona 08019, Spain
关键词
melon; AFLP; RFLP; RAPD; genetic similarity;
D O I
10.1007/s001220051553
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Three different types of molecular markers, RAPD, AFLP and RFLP were used to measure genetic diversity among six genotypes of Cucumis melo L. Each line represented a different melon genotype: Piel de Sapo, Ogen, PI161375, PI414723, Agrestis and C105. A number of polymorphic RAPD, AFLP and RFLP bands were scored on all materials and the genetic similarity measured. Clustering analysis performed with the three types of markers separated the genotypes into two main groups: (I) the sweet type, cultivated melons and (2) the exotic type, not cultivated melons. While the data obtained suggest that all three types of markers are equally informative, AFLPs showed the highest efficiency in detecting polymorphism.
引用
收藏
页码:860 / 864
页数:5
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