Lymphocyte reconstitution following allogeneic hematopoietic stem cell transplantation:: a retrospective study including 148 patients

被引:52
作者
Heining, C.
Spyridonidis, A.
Bernhardt, E.
Schulte-Moenting, J.
Behringer, D.
Gruellich, C.
Jakob, A.
Bertz, H.
Finke, J.
机构
[1] Univ Freiburg, Dept Hematol Oncol, D-79106 Freiburg, Germany
[2] Univ Freiburg, Dept Med Biometry & Stat, D-79106 Freiburg, Germany
[3] Klinikum Offenburg, Dept Hematol Oncol, Offenburg, Germany
关键词
hematopoietic stem cell transplantation; lymphocyte reconstitution; reduced-intensity conditioning;
D O I
10.1038/sj.bmt.1705648
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Here we investigated the influence of parameters known before hematopoietic stem cell transplantation (HSCT) as well as the relevance of graft-versus-host disease (GvHD) and cytomegalovirus (CMV) reactivation on post transplant lymphocyte reconstitution in 148 patients treated in our institution between 1996 and 2003. Median patient age was 42 (19 - 68) years, HSCT followed standard high dose (n = 91) or reduced-intensity conditioning regimens (n = 57) with bone marrow (BM, n = 67) or peripheral blood stem cells (PBSC, n = 81) from related (n = 71) or unrelated (n = 77) donors. In the. rst months, we observed a partially faster reconstitution of CD3(+)4(+), CD3(+)8(+) and CD4(+)45RA(+) T cells in patients following peripheral blood stem cell transplantation when compared to bone marrow transplantation. Prolonged CD3(+)4(+) and CD4(+)45RA(+) lymphopenia was noted after unrelated donor HSCT and GvHD prophylaxis containing anti-T- lymphocyte globulin. Lymphocyte subset counts in patients older than the median age were comparable to those in patients transplanted at a younger age and not influenced by the conditioning regimen. CD3(+)8(+) T cell reconstitution was strongly correlated with CMV reactivation, but not significantly affected by CMV serostatus before HSCT. Incidence or extent of GvHD did not significantly in. uence lymphocyte reconstitution. Therefore, the source of graft is the most predictive parameter in early lymphocyte reconstitution, but the differences in lymphocyte recovery completely resolved within the first year after HSCT.
引用
收藏
页码:613 / 622
页数:10
相关论文
共 46 条
[1]  
Abrahamsen IW, 2005, HAEMATOLOGICA, V90, P86
[2]   Immune restoration following hematopoietic stem cell transplantation: an evolving target [J].
Auletta, JJ ;
Lazarus, HM .
BONE MARROW TRANSPLANTATION, 2005, 35 (09) :835-857
[3]   Early reconstitution of the T-cell repertoire after non-myeloablative peripheral blood stem cell transplantation is from post-thymic T-cell expansion and is unaffected by graft-versus-host disease or mixed chimaerism [J].
Bahceci, E ;
Epperson, D ;
Douek, DC ;
Melenhorst, JJ ;
Childs, RC ;
Barrett, AJ .
BRITISH JOURNAL OF HAEMATOLOGY, 2003, 122 (06) :934-943
[4]   Non-myeloablative stem cell transplants [J].
Barrett, J ;
Childs, R .
BRITISH JOURNAL OF HAEMATOLOGY, 2000, 111 (01) :6-17
[5]   Quantitative lymphocyte subset reconstitution after allogeneic hematopoietic transplantation from matched related donors with CD34+ selected PBPC grafts, unselected PBPC grafts or BM grafts [J].
Behringer, D ;
Bertz, H ;
Schmoor, C ;
Berger, C ;
Dwenger, A ;
Finke, J .
BONE MARROW TRANSPLANTATION, 1999, 24 (03) :295-302
[6]   Allogeneic peripheral blood stem cell transplantation in patients with advanced hematologic malignancies: A retrospective comparison with marrow transplantation [J].
Bensinger, WI ;
Clift, R ;
Martin, P ;
Appelbaum, FR ;
Demirer, T ;
Gooley, T ;
Lilleby, K ;
Rowley, S ;
Sanders, J ;
Storb, R ;
Buckner, CD .
BLOOD, 1996, 88 (07) :2794-2800
[7]   GUIDELINE FOR FLOW CYTOMETRIC IMMUNOPHENOTYPING - A REPORT FROM THE NATIONAL-INSTITUTE-OF-ALLERGY-AND-INFECTIOUS-DISEASES, DIVISION OF AIDS [J].
CALVELLI, T ;
DENNY, TN ;
PAXTON, H ;
GELMAN, R ;
KAGAN, J .
CYTOMETRY, 1993, 14 (07) :702-715
[8]  
CHAN EYT, 1994, ASIAN PAC J ALLERGY, V12, P117
[9]   Engraftment kinetics after nonmyeloablative allogeneic peripheral blood stem cell transplantation: Full donor T-cell chimerism precedes alloimmune responses [J].
Childs, R ;
Clave, E ;
Contentin, N ;
Jayasekera, D ;
Hensel, N ;
Leitman, S ;
Read, EJ ;
Carter, C ;
Bahceci, E ;
Young, NS ;
Barrett, AJ .
BLOOD, 1999, 94 (09) :3234-3241
[10]   Dendritic cells - Dendritic cell recovery after autologous stem cell transplantation [J].
Damiani, D ;
Stocchi, R ;
Masolini, P ;
Michelutti, A ;
Sperotto, A ;
Geromin, A ;
Skert, C ;
Cerno, M ;
Michieli, M ;
Baccarani, M ;
Fanin, R .
BONE MARROW TRANSPLANTATION, 2002, 30 (05) :261-266