Mechanisms involved in vitamin E transport by primary enterocytes and in vivo absorption

被引:76
作者
Anwar, Kamran
Iqbal, Jahangir
Hussain, M. Mahmood [1 ]
机构
[1] Suny Downstate Med Ctr, Dept Anat & Cell Biol, Brooklyn, NY 11203 USA
[2] Suny Downstate Med Ctr, Dept Pediat, Brooklyn, NY 11203 USA
[3] Suny Downstate Med Ctr, Sch Grad Studies, Program Mol & Cellular Biol, Brooklyn, NY 11203 USA
关键词
lipoproteins; tocopherol; HDL; chylomicrons; oleic acid;
D O I
10.1194/jlr.M700207-JLR200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It is generally believed that vitamin E is absorbed along with chylomicrons. However, we previously reported that human colon carcinoma Caco-2 cells use dual pathways, apolipoprotein B (apoB)-lipoproteins and HDLs, to transport vitamin E. Here, we used primary enterocytes and rodents to identify in vivo vitamin E absorption pathways. Uptake of [H-3]alpha-tocopherol by primary rat and mouse enterocytes increased with time and reached a maximum at 1 h. In the absence of exogenous lipid supply, these cells secreted vitamin E with HDL. Lipids induced the secretion of vitamin E with intermediate density lipoproteins, and enterocytes supplemented with lipids and oleic acid secreted vitamin E with chylomicrons. The secretion of vitamin E with HDL was not affected by lipid supply but was enhanced when incubated with HDL. Microsomal triglyceride transfer protein inhibition reduced vitamin E secretion with chylomicrons without affecting its secretion with HDL. Enterocytes from Mttp-deficient mice also secreted less vitamin E with chylomicrons. In vivo absorption of [H-3]alpha-tocopherol by mice after poloxamer 407 injection to inhibit lipoprotein lipase revealed that vitamin E was associated with triglyceride-rich lipoproteins and small HDLs containing apoB-48 and apoA-I. These studies indicate that enterocytes use two pathways for vitamin E absorption. Absorption with chylomicrons is the major pathway of vitamin E absorption. The HDL pathway may be important when chylomicron assembly is defective and can be exploited to deliver vitamin E without increasing fat consumption.
引用
收藏
页码:2028 / 2038
页数:11
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