cDNA cloning and expression analysis of the murine ribonuclease L inhibitor

被引：9

作者：

De Coignac, AB ^{[1
]}

Bisbal, C ^{[1
]}

Lebleu, B ^{[1
]}

Salehzada, T ^{[1
]}

机构：

[1] IGMM, CNRS, UMR 5535, F-34293 Montpellier 5, France

来源：

GENE | 1998年 / 209卷 / 1-2期

关键词：

interferon; RNase L; 2-5A system; nucleotide sequence; expression;

D O I：

10.1016/S0378-1119(98)00040-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The 2-5A/RNase L system is one of the pathways induced by interferon (IFN). It plays a major role in the antiviral and antiproliferative activities of IFNs. Recently, we have shown that the activity of the RNase L could be inhibited by a proteic inhibitor, the RNase L Inhibitor (RLI). Human RLI (Hu-RLI) was cloned and characterized. We describe here the isolation and characterization of the cDNA encoding the murine RLI (Mu-RLI). Hu-RLI and Mu-RLI protein have 98% amino acid identity. Mu-RLI is functionally homologous to Hu-RLI, and all the structural features and amino acid sequence motifs of Hu-RLI are conserved in Mu-RLI. Moreover, reticulocyte lysate translated Mu-RLI protein is also able to inhibit 2-5A binding on 2-5A-dependent RNAse-L. Northern blot analysis revealed that Mu-RLI cDNA hybridizes with one mRNA of 3.5 kb except for the testis where two mRNA of 3.5 and 2.1 kb, respectively, are detected, suggesting a tissue-specific regulation. (C) 1998 Elsevier Science B.V.

引用

页码：149 / 156

页数：8

共 28 条

[1]

[Anonymous], 1982, MOL CLONING LAB MANU

[2] Chromosomal localization and expression pattern of the RNase L inhibitor gene [J].