Extensive polymorphism and different evolutionary patterns of intron 2 were identified in the HLA-DQB1 gene

被引：15

作者：

Fu, YG ^{[1
]}

Liu, ZH ^{[1
]}

Lin, JH ^{[1
]}

Chen, WM ^{[1
]}

Jia, ZJ ^{[1
]}

Pan, DJ ^{[1
]}

Xu, AL ^{[1
]}

机构：

[1] Zhongshan Univ, Dept Biochem, Key Lab Genet Engn MOE, Coll Life Sci, Guangzhou 510275, Peoples R China

来源：

IMMUNOGENETICS | 2003年 / 54卷 / 11期

关键词：

HLA; DQB1; intron; polymorphism; evolution;

D O I：

10.1007/s00251-002-0523-z

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Exon 2 and intron 2 of the HLA DQB1 gene from 20 individuals were cloned and sequenced and eight alleles were obtained. Based on our analysis, the nucleotide diversity of the 5' end of intron 2 was higher than the synonymous nucleotide diversity of exon 2, which may be due to the lower GC content and the 'hitch-hiking effect'. In contrast, the opposite phenomenon was observed for the 3' end of intron 2, which may be the result of the recombination between the 3' end and 5' end of intron 2 and the subsequent genetic drift. The results indicated that different regions of intron 2 in the DQB1 gene had different evolutionary patterns.

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收藏

页码：761 / 766

页数：6

相关论文

共 28 条

[1]

APPLE RJ, 1996, HLA MHC GENES MOL FU, P101

[2] THE MYTH OF EVE - MOLECULAR-BIOLOGY AND HUMAN ORIGINS [J].

AYALA, FJ .

SCIENCE, 1995, 270 (5244) :1930-1936

[3] The evolution of human populations: A molecular perspective [J].

Ayala, FJ ;

Escalante, AA .

MOLECULAR PHYLOGENETICS AND EVOLUTION, 1996, 5 (01) :188-201

[4] Complete sequence and gene map of a human major histocompatibility complex [J].

Beck, S ;

Geraghty, D ;

Inoko, H ;

Rowen, L ;

Aguado, B ;

Bahram, S ;

Campbell, RD ;

Forbes, SA ;

Guillaudeux, T ;

Hood, L ;

Horton, R ;

Janer, M ;

Jasoni, C ;

Madan, A ;

Milne, S ;

Neville, M ;

Oka, A ;

Qin, S ;

Ribas-Despuig, G ;

Rogers, J ;

Shiina, T ;

Spies, T ;

Tamiya, G ;

Tashiro, H ;

Trowsdale, J ;

Vu, Q ;

Williams, L ;

Yamazaki, M .

NATURE, 1999, 401 (6756) :921-923

[5] Evolutionary dynamics of non-coding sequences within the class II region of the human MHC [J].

Beck, S ;

Abdulla, S ;

Alderton, RP ;

Glynne, RJ ;

Gut, IG ;

Hosking, LK ;

Jackson, A ;

Kelly, A ;

Newell, WR ;

Sanseau, P ;

Radley, E ;

Thorpe, KL ;

Trowsdale, J .

JOURNAL OF MOLECULAR BIOLOGY, 1996, 255 (01) :1-13

[6] Recent origin of HLA-DRB1 alleles and implications for human evolution [J].

Bergström, TF ;

Josefsson, A ;

Erlich, HA ;

Gyllensten, U .

NATURE GENETICS, 1998, 18 (03) :237-242

[7] 3-DIMENSIONAL STRUCTURE OF THE HUMAN CLASS-II HISTOCOMPATIBILITY ANTIGEN HLA-DR1 [J].

BROWN, JH ;

JARDETZKY, TS ;

GORGA, JC ;

STERN, LJ ;

URBAN, RG ;

STROMINGER, JL ;

WILEY, DC .

NATURE, 1993, 364 (6432) :33-39

[8] Locus-specific conservation of the HLA class I introns by intra-locus homogenization [J].

Cereb, N ;

Hughes, AL ;

Yang, SY .

IMMUNOGENETICS, 1997, 47 (01) :30-36

[9]

CHARLESWORTH B, 1993, GENETICS, V134, P1289

[10] ENHANCED IMMUNOLOGICAL SURVEILLANCE IN MICE HETEROZYGOUS AT H-2 GENE COMPLEX [J].

DOHERTY, PC ;

ZINKERNAGEL, RM .

NATURE, 1975, 256 (5512) :50-52