Molecular cloning and characterization of starch-branching enzyme II from potato

Full-length cDNA for starch branching enzyme (SBE) II of potato was isolated and sequenced. In potato, similary to most other investigated plants, the SEE-II isoform differs from SEE-I by having an acidic amino-terminal extension and a shorter carboxyterminus. Two forms of SEE-II, migrating as 98 and 95 kDa proteins in 6% SDS-polyacrylamide gels, were associated to tuber starch. The latter form was 16 amino acids shorter in the amino terminus. Transcript of SEE-II was present in leaf tissue, whereas significant expression was not seen in tubers. On the other hand, a significant amount of SEE-I transcript was detected in tuber tissue but not in leaves.