Genome mapping by fluorescent fingerprinting

被引：52

作者：

Gregory, SG ^{[1
]}

Howell, GR ^{[1
]}

Bentley, DR ^{[1
]}

机构：

[1] Sanger Ctr, Hinxton CB10 1SA, Cambs, England

来源：

GENOME RESEARCH | 1997年 / 7卷 / 12期

关键词：

D O I：

10.1101/gr.7.12.1162

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The construction of sequence-ready maps of overlapping genomic clones is central to large-scale genome sequencing. We have implemented a method for fluorescent fingerprinting of bacterial clones to assemble contig maps. The method utilizes three spectrally distinct fluorescently tagged dideoxy ATPs to specifically label the HindIII termini in HindIII and Sau3Al restriction digests of clones that are multiplexed prior to electrophoresis and data collection. There is excellent reproducibility of raw data, improved resolution of large Fragments, and concordance between the results obtained using this and the equivalent radioactive protocol. This method also allows detection of smaller overlaps between clones when compared to the analysis of restriction digests on nondenaturing agarose gels.

引用

页码：1162 / 1168

页数：7

共 19 条

[1]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[2] A HIGH-RESOLUTION, FLUORESCENCE-BASED, SEMIAUTOMATED METHOD FOR DNA FINGERPRINTING [J].