The joys of in vitro selection: chemically dressing oligonucleotides to satiate protein targets

被引：86

作者：

Eaton, BE ^{[1
]}

机构：

[1] NeXstar Pharmaceut Inc, Boulder, CO 80301 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 1997年 / 1卷 / 01期

关键词：

D O I：

10.1016/S1367-5931(97)80103-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oligonucleotide in vitro selection provides surprisingly specific aptamers to protein targets. Synthetic chemistries for modifying nucleotides have been developed to enhance aptamer binding affinity. The diversity of nucleosides amenable to either triphosphate synthesis or phosphoramidite activation is now sufficiently broad to rival any oligomeric combinatorial library.

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页码：10 / 16

页数：7

相关论文

共 65 条

[1] Some biochemical thoughts on the RNA world [J].

Bloch, K .

CHEMISTRY & BIOLOGY, 1996, 3 (05) :405-407

[2] SELECTION AND CHARACTERIZATION OF RNAS REPLICATED BY Q-BETA REPLICASE [J].

BROWN, D ;

GOLD, L .

BIOCHEMISTRY, 1995, 34 (45) :14775-14782

[3] SYNTHETIC RIBOZYMES AND THE FIRST DEOXYRIBOZYME [J].

BURGSTALLER, P ;

FAMULOK, M .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1995, 34 (11) :1189-1192

[4] Structural probing and damage selection of citrulline- and arginine-specific RNA aptamers identify base positions required for binding [J].

Burgstaller, P ;

Kochoyan, M ;

Famulok, M .

NUCLEIC ACIDS RESEARCH, 1995, 23 (23) :4769-4776

[5]

CIESIOLKA J, 1995, RNA, V1, P538

[6] Porphyrin metalation catalyzed by a small RNA molecule [J].

Conn, MM ;

Prudent, JR ;

Schultz, PG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (29) :7012-7013

[7] In vitro selection methodologies to probe RNA function and structure [J].

Conrad, RC ;

Baskerville, S ;

Ellington, AD .

MOLECULAR DIVERSITY, 1995, 1 (01) :69-78

[8] SYNTHESIS OF 2'-DEOXYURIDINE NUCLEOSIDES WITH APPENDED 5-POSITION CARBONYL CROSS-LINKING GROUPS [J].

CROUCH, GJ ;

EATON, BE .

NUCLEOSIDES & NUCLEOTIDES, 1994, 13 (04) :939-944

[9] A DNA METALLOENZYME WITH DNA-LIGASE ACTIVITY [J].

CUENOUD, B ;

SZOSTAK, JW .

NATURE, 1995, 375 (6532) :611-614

[10] RNA world: Functional diversity in a nucleoside by carboxyamidation of uridine [J].

Dewey, TM ;

Zyzniewski, MC ;

Eaton, BE .

NUCLEOSIDES & NUCLEOTIDES, 1996, 15 (10) :1611-1617

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