Hepatocyte nuclear factors 1α and 4α control expression of proline oxidase in adult liver

被引：13

作者：

Kamiya, A

Inoue, Y

Kodama, T

Gonzalez, FJ

机构：

[1] NCI, Lab Metab, NIH, Bethesda, MD 20892 USA

[2] Univ Tokyo, Lab Syst Biol & Med, RCAST 34, Tokyo 1538904, Japan

来源：

FEBS LETTERS | 2004年 / 578卷 / 1-2期

基金：

日本学术振兴会;

关键词：

amino acid metabolism; liver-enriched; transcription factor; gene expression; proline oxidase;

D O I：

10.1016/j.febslet.2004.10.070

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adult liver functions are regulated by several hepatocyte nuclear factors (HNFs). HNF4alpha and HNF1alpha are involved in metabolic functions in the liver. The expression of proline oxidase (PO) and proline dehydrogenase was downregulated in the HNF4alpha liver-specific null mice. In addition, the expression of PO was also diminished in the liver derived from HNF1alpha-null mice. The -160 bp proximal promoter region of the PO gene has two HNF4alpha and HNF1alpha-binding consensus sites. Transactivation, electrophoretic mobility shift and chromatin immunoprecipitation studies revealed that these regions are important for PO promoter activity. These results suggested that HNF4alpha and HNF1alpha regulate proline metabolism in adult liver. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：63 / 68

页数：6

共 22 条

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