Intracellular Ca2+ imaging for micropatterned cardiac myocytes

被引：25

作者：

Kaji, H ^{[1
]}

Takoh, K ^{[1
]}

Nishizawa, M ^{[1
]}

Matsue, T ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Engn, Dept Biomol Engn, Sendai, Miyagi 9808579, Japan

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2003年 / 81卷 / 06期

关键词：

cardiac myocytes; microcontact printing (mu CP); pattering; confocal microscope; Ca2+ imaging;

D O I：

10.1002/bit.10521

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The patterning of cardiac myocytes on a micron scale (similar to5 mum) was achieved by microcontact printing of fibronectin onto a hydrophobically pretreated glass substrate. The patterned cardiac myocytes conjugated with each other by forming a gap junction, as judged from the synchronized Ca2+ transition over the pattern, and thus simultaneously contracted. The dynamic change of the Ca2+ concentration within the patterned tissue was analyzed quantitatively during successive contraction and relaxation using a Nipkow-type high-speed confocal microscope. (C) 2003 Wiley Periodicals, Inc.

引用

页码：748 / 751

页数：4

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