The NB protein of influenza B virus is not necessary for virus replication in vitro

被引：59

作者：

Hatta, M

Kawaoka, Y ^{[1
]}

机构：

[1] Univ Wisconsin, Sch Vet Med, Dept Pathobiol Sci, Madison, WI 53706 USA

[2] Univ Tokyo, Inst Med Sci, Tokyo 1088639, Japan

[3] Japan Sci & Technol Corp, Core Res Evaluat Sci & Technol, Saitama 3320012, Japan

来源：

JOURNAL OF VIROLOGY | 2003年 / 77卷 / 10期

关键词：

D O I：

10.1128/JVI.77.10.6050-6054.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The NB protein of influenza B virus is thought to function as an ion channel and therefore would be expected to have an essential function in viral replication. Because direct evidence for its absolute requirement in the viral life cycle is lacking, we generated NB knockout viruses by reverse genetics and tested their growth properties both in vitro and in vivo. Mutants not expressing NB replicated as efficiently as the wild-type virus in cell culture, whereas in mice they showed restricted growth compared with findings for the wild-type virus. Thus, the NB protein is not essential for influenza B virus replication in cell culture but promotes efficient growth in mice.

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页码：6050 / 6054

页数：5

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