Purification and characterization of a novel glycoprotein from wheat germ water-soluble extracts

被引：22

作者：

Zhu, KX ^{[1
]}

Zhou, HM ^{[1
]}

机构：

[1] So Yangtze Univ, Sch Food Sci & Technol, Wuxi 214036, Jiangsu, Peoples R China

来源：

PROCESS BIOCHEMISTRY | 2005年 / 40卷 / 3-4期

关键词：

wheat germ; glycoprotein; water-soluble extracts; SDS-PAGE; beta-elimination;

D O I：

10.1016/j.procbio.2004.06.030

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel glycoprotein, referred to as WGGP, was isolated from wheat germ water-soluble extracts, and purified by ion-exchange and gel filtration column chromatography. The homogeneity of WGGP was demonstrated by gel filtration on Sepharose CL-6B. The galycoprotein contains 56.4% of protein, rich in glutamic acid, asparagic acid, alanine, glycine, valine and leucine. Mannose accounted for 94% of the neutral sugar moiety, and traces of arabinose, xylose, glucose and galactose were found in WGGP. The molecular weight was estimated to be about 40,000 on SDS-PAGE. The existence of O-glycosidic linkage in WGGP was demonstrated with a beta-elimination reaction. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：1469 / 1474

页数：6

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