Development and evaluation of a nested PCR based on spherical body protein 2 gene for the diagnosis of Babesia bovis infection

被引：32

作者：

AbouLaila, Mahmoud ^{[1
]}

Yokoyama, Naoaki ^{[1
]}

Igarashi, Ikuo ^{[1
]}

机构：

[1] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan

来源：

VETERINARY PARASITOLOGY | 2010年 / 169卷 / 1-2期

关键词：

Nested PCR; Spherical body protein 2 gene; Babesia bovis; POLYMERASE-CHAIN-REACTION; ERYTHROCYTE-MEMBRANE; BOOPHILUS-MICROPLUS; BIGEMINA; PARASITES; CATTLE; DNA; AMPLIFICATION; THEILERIA; ANTIGENS;

D O I：

10.1016/j.vetpar.2009.12.013

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

We developed and evaluated a nested PCR assay for the diagnosis of Babesia bovis infection in cattle based on the spherical body protein 2 gene (SBP2) from B. bovis. The specificity and sensitivity of the test were compared with the B. bovis RAP-1 gene nPCR. The SBP2 primers have specificities of 100% for B. bovis DNA. The sensitivity of the SBP2 nPCR to B. bovis from the in vitro cultured parasites was higher than that of the B. bovis RAP-1 gene nPCR, and a parasitemia as low as 10(-8)% was detected. The sensitivity of the SBP2 nPCR to B. bovis-diluted genomic DNA was also higher than that of B. bovis RAP-1 gene nPCR, and as little as 1 fg per test detected. For field applications, the sensitivity to a total of 145 field samples from Ghana, Mongolia, and Brazil was evaluated. The nPCR assay of spherical body protein-2 gene detected 87.6% (127/145), while B. bovis RAP-1 gene nPCR detected 37.2% (51/145) of the total samples examined. This nPCR assay provides a good diagnostic tool for the laboratory diagnostic assessment of B. bovis infection in cattle worldwide. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：45 / 50

页数：6

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