Dibenzothiophene biodesulfurization in resting cell conditions by aerobic bacteria

被引:72
作者
Caro, Ainhoa [1 ]
Boltes, Karina [1 ]
Leton, Pedro [1 ]
Garcia-Calvo, Eloy [1 ]
机构
[1] Univ Alcala de Henares, Fac Ciencias, Dpto Quim Analit & Ingn Quim, Madrid 28871, Spain
关键词
biodesulfurization; dibenzothiophene; resting cell; mass transfer; pseudomonas sp CECT 5279; Rhodococcus sp IGTS8;
D O I
10.1016/j.bej.2007.01.013
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Rhodococcus erythropolis IGTS8 and the genetically modified strain Pseudomonas putida CECT 5279 are effectively capable to carry out the oxidative non-destructive desulfurization of dibenzothiophene (DBT), called 4S pathway, so that sulfur is released after four enzymatic steps, without carbon skeleton breakdown. Biodesulfurization (BDS) process yields were studied both in aqueous and biphasic media, with hexadecane as model oil. All batch assays were carried out at Erlenmeyer flasks scale, under different experimental conditions such as oil fraction phase percentages, substrate concentrations and cellular densities, using resting cell as operation mode. Providing that cell densities were not too high, both biocatalysts achieved better DBT conversion with higher biomass concentrations and lower oil fractions and DBT concentrations. Comparing aqueous and biphasic media it has been also proved that P. putida CECT 5279 strain was more sensitive for DBT mass transfer limitation, determining the preferable use in biphasic systems of IGTS8 to practical BDS process as well. Furthermore, the experimental results suggested there could be inhibitions effects by product accumulation in aqueous media, but these effects were not so clear in biphasic condition, because 2-hydroxybiphenyl (HBP) oil-water partition coefficient is very high. (C) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:191 / 197
页数:7
相关论文
共 29 条
[1]   Multi-criteria comparison of resting cell activities of bacterial strains selected for biodesulfurization of petroleum compounds [J].
Abbad-Andaloussi, S ;
Lagnel, C ;
Warzywoda, M ;
Monot, F .
ENZYME AND MICROBIAL TECHNOLOGY, 2003, 32 (3-4) :446-454
[2]   Biodesulfurisation of DBT with Pseudomonas putida CECT5279 by resting cells:: Influence of cell growth time on reducing equivalent concentration and HpaC activity [J].
Alcon, A ;
Santos, VE ;
Martin, AB ;
Yustos, P ;
Garcia-Ochoa, F .
BIOCHEMICAL ENGINEERING JOURNAL, 2005, 26 (2-3) :168-175
[3]   Modeling the production of a Rhodococcus erythropolis IGTS8 biocatalyst for DBT biodesulfurization:: Influence of media composition [J].
del Olmo, CH ;
Alcon, A ;
Santos, VE ;
Garcia-Ochoa, F .
ENZYME AND MICROBIAL TECHNOLOGY, 2005, 37 (02) :157-166
[4]   Production of a Rhodococcus erythropolis IGTS8 biocatalyst for DBT biodesulfurization:: influence of operational conditions [J].
del Olmo, CH ;
Santos, VE ;
Alcon, A ;
Garcia-Ochoa, F .
BIOCHEMICAL ENGINEERING JOURNAL, 2005, 22 (03) :229-237
[5]  
GALLAGHER JR, 1993, FEMS MICROBIOL LETT, V107, P31, DOI 10.1111/j.1574-6968.1993.tb05999.x
[6]   Designing recombinant Pseudomonas strains to enhance biodesulfurization [J].
Gallardo, ME ;
Ferrandez, A ;
DeLorenzo, V ;
Garcia, JL ;
Diaz, E .
JOURNAL OF BACTERIOLOGY, 1997, 179 (22) :7156-7160
[7]   Bio-desulfurization of model organo-sulfur compounds and hydrotreated diesel - Experiments and modeling [J].
Guchhait, S ;
Biswas, D ;
Bhattacharya, P ;
Chowdhury, R .
CHEMICAL ENGINEERING JOURNAL, 2005, 112 (1-3) :145-151
[8]  
GUOBIN S, 2006, BIOCH ENG J, V27, P305
[9]   High cell density culture of Rhodococcus rhodochrous by pH-stat feeding and dibenzothiophene degradation [J].
Honda, H ;
Sugiyama, H ;
Saito, I ;
Kobayashi, T .
JOURNAL OF FERMENTATION AND BIOENGINEERING, 1998, 85 (03) :334-338
[10]   Modeling of DBT biodegradation behaviors by resting cells of Gordonia sp WQ-01 and its mutant in oil-water dispersions [J].
Jia, X ;
Wen, JP ;
Sun, ZP ;
Caiyin, QG ;
Xie, SP .
CHEMICAL ENGINEERING SCIENCE, 2006, 61 (06) :1987-2000