Nanowiring of the catalytic site of novel molecular enzyme-metal hybrids to electrodes

Directed deposition of silver on the surface of single, soluble enzyme molecules was made possible by controlled conjugation of new silver-reducing moieties to the surface of the enzyme molecule and their reaction with silver ions. Molecular enzyme-silver hybrids carrying low or high crystalline silver deposited on their surface were obtained, retaining their solubility and enzymatic activity. The feasibility of "wiring" of the active site of glucose oxidase-silver hybrid thus obtained to platinum electrode was readily demonstrated enabling glucose determination in the absence of oxygen.