Double (fluorescent and spin) sensors for detection of reactive oxygen species in the thylakoid membrane

被引：81

作者：

Kálai, T

Hideg, É

Vass, I

Hideg, K

机构：

[1] Univ Pecs, Inst Organ & Med Chem, H-7643 Pecs, Hungary

[2] Hungarian Acad Sci, Biol Res Ctr, Inst Plant Biol, H-6701 Szeged, Hungary

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 1998年 / 24卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

ESR spectroscopy; fluorescence; photoinhibition; singlet oxygen; free radical;

D O I：

10.1016/S0891-5849(97)00339-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of dansylated sterically hindered amines designed to trapping reactive oxygen species, were synthesized. Compounds were tested in isolated thylakoid membranes subjected to photoinhibition by excess photosynthetically active radiation (400-700 nm). DanePy showed good selectivity for singlet oxygen and the formation of nitroxide was detected by appearance of ESR signal and quenching fluorescence. (C) 1998 Elsevier Science Inc.

引用

页码：649 / 652

页数：4

共 18 条

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