Neurotoxin gene clusters in Clostridium botulinum type A strains:: Sequence comparison and evolutionary implications

被引：61

作者：

Dineen, SS ^{[1
]}

Bradshaw, M ^{[1
]}

Johnson, EA ^{[1
]}

机构：

[1] Univ Wisconsin, Inst Food Res, Dept Food Microbiol & Toxicol, Madison, WI 53706 USA

来源：

CURRENT MICROBIOLOGY | 2003年 / 46卷 / 05期

关键词：

D O I：

10.1007/s00284-002-3851-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The nucleotide sequence of the hemagglutinin (ha) genes and the transcriptional regulator botR gene were determined in type A Clostridium botulinum strain 62A, and the complete nucleotide sequence of the botulinum neurotoxin (BoNT) gene cluster was determined in strain Hall A-hyper. Comparison of the BoNT/A gene clusters revealed only two nucleotide differences between the two strains. The nucleotide sequences of the regions flanking the BoNT clusters were also determined in strains 62A, Hall A-hyper, and type A(B) strain NCTC 2916. The regions upstream of the BoNT/A clusters in the type A strains shared marked homology with the region upstream of the silent BoNT/B cluster in the A(B) strain, indicating a similar evolutionary origin. The region downstream of the BoNT/A cluster in type A strains encodes putative insertion sequence (IS) elements with multiple internal mutations. These IS elements may have played a role in neurotoxin gene transfer within the host genome and to other Clostridium species.

引用

页码：345 / 352

页数：8

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