Glypican-3 is overexpressed in human hepatocellular carcinoma

被引:117
作者
Sung, YK
Hwang, SY
Park, MK
Farooq, M
Han, IS
Bae, HI
Kim, JC
Kim, M [1 ]
机构
[1] Kyungpook Natl Univ, Sch Med, Dept Immunol, Chung Gu, Taegu 700422, South Korea
[2] Kyungpook Natl Univ, Sch Med, Dept Pathol, Chung Gu, Taegu 700422, South Korea
来源
CANCER SCIENCE | 2003年 / 94卷 / 03期
关键词
D O I
10.1111/j.1349-7006.2003.tb01430.x
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
To identify candidate genes that could be used as diagnostic and therapeutic targets for hepatocellular carcinoma (HCC), we searched for the genes that are overexpressed in HCC by combining representational difference analysis and microarray. Genes such as glypican-3 (GPC3), insulin-like growth factor 2, long-chain fatty-acid-coenzyme A ligase 4, farnesyl diphosphate synthase were frequently identified in our screening. Northern blot analysis with these four genes confirmed their overexpression in HCC. Among them we found that GPC3 transcript is upregulated in six out of seven cases of HCC. Immunoblot and immumohistochemical staining using polyclonal anti-GPC3 antibodies further confirmed that GPC3 protein is indeed increased in HCC tumor samples. We also found that GPC3 is secreted into culture media from cell lines derived from HCC. We conclude that GPC3 is a good molecular marker for HCC.
引用
收藏
页码:259 / 262
页数:4
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