Nigella sativa seed extracts enhance glucose-induced insulin release from rat-isolated Langerhans islets

被引:75
作者
Rchid, H
Chevassus, H
Nmila, R
Guiral, C
Petit, P
Chokaïri, M
Sauvaire, Y
机构
[1] Univ Montpellier 2, Lab Rech Subst Nat Vegetales, UPRES EA 1677, F-34095 Montpellier 05, France
[2] CNRS, UMR 5160, Ctr Pharmacol & Biotechnol Sante, Montpellier, France
[3] Univ Chouaib Doukkali, Fac Sci, Dept Sci Via, El Jadida, Morocco
[4] Univ Mohammed 5, Lab Physiol Vegetale, Rabat, Morocco
关键词
diabetes; insulin secretion; Nigella sativa; rat islets; seed extract; seed fractionation;
D O I
10.1111/j.1472-8206.2004.00275.x
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Nigella sativa L. 'Black cumin' (Ranunculaceae) is one of the plants commonly used in Moroccan folk medicine for treatment of various ailments including diabetes mellitus. The present study was undertaken to investigate the effect of different N. sativa seed extracts on insulin secretion. Different fractions of the seed were prepared: the defatted fraction (FIR 11), which was divided into two subfractions: the first (HR III) containing acidic and neutral compounds and the second (HR IV) containing basic compounds. The insulin secretory effects of these extracts were evaluated individually at different concentrations (0.01, 0.1, 1 and 5 mg/mL), in vitro in isolated rat pancreatic islets in the presence of 8.3 mmol/L glucose. The results show that addition of the defatted whole extract or of the basic subfraction of the seed in the incubation medium significantly increased glucose-induced insulin release from the islets. In the case of the acidic and neutral subfraction, the stimulatory effect was observed only for the higher concentration (5 mg/mL). However, a clear concentration-dependent increase in insulin release from isolated pancreatic islets was observed for the basic subfraction. Our data show that the antidiabetic properties of N. sativa seeds may be, at least partly, mediated by stimulated insulin release, and that the basic subfraction largely contributes to this stimulatory effect. Further phytochemical studies are underway in order to isolate the pharmacological compound(s) responsible for the insulinotropic effect of N. sativa seeds.
引用
收藏
页码:525 / 529
页数:5
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