Cooxidation of naphthalene and other polycyclic aromatic hydrocarbons by the nitrifying bacterium, Nitrosomonas europaea

被引:58
作者
Chang, SW [1 ]
Hyman, MR
Williamson, KJ
机构
[1] Kyonggi Univ, Dept Civil & Environm Engn, Suwon, South Korea
[2] N Carolina State Univ, Dept Microbiol, Raleigh, NC 27695 USA
[3] Oregon State Univ, Dept Civil Construct & Environm Engn, Corvallis, OR 97331 USA
关键词
D O I
10.1023/A:1022811430030
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The soil nitrifying bacterium Nitrosomonas europaea has shown the ability to transform cometabolically naphthalene as well as other 2- and 3- ringed polycyclic aromatic hydrocarbons (PAHs) to more oxidized products. All of the observed enzymatic reactions were inhibited by acetylene, a selective inhibitor of ammonia monooxygenase (AMO). A strong inhibitory effect of naphthalene on ammonia oxidation by N. europaea was observed. Naphthalene was readily oxidized by N. europaea and 2- naphthol was detected as a major product (85%) of naphthalene oxidation. The maximum naphthol production rate was 1.65 nmole/mg protein-min in the presence of 240 muM naphthalene and 10 mM NH4+. Our results demonstrate that the oxidation between ammonia and naphthalene showed a partial competitive inhibition. The relative ratio of naphthalene and ammonia oxidation, depending on naphthalene concentrations, demonstrated that the naphthalene was oxidized 2200-fold slower than ammonia at lower concentration of naphthalene (15 muM) whereas naphthalene was oxidized only 100-fold slower than ammonia oxidation. NH4+- and N2H4-dependent O-2 uptake measurement demonstrated irreversible inhibitory effects of the naphthalene and subsequent oxidation products on AMO and HAO activity.
引用
收藏
页码:373 / 381
页数:9
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