Investigation on response of the metabolites in tricarboxylic acid cycle of Escherichi coli and Pseudomonas aeruginosa to antibiotic perturbation by capillary electrophoresis

被引：17

作者：

Gao, Peng ^{[1
]}

Shi, Chunyun ^{[1
]}

Tian, Jing ^{[1
]}

Shi, Xianzhe ^{[1
]}

Yuan, Kailong ^{[1
]}

Lu, Xin ^{[1
]}

Xu, Guowang ^{[1
]}

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2007年 / 44卷 / 01期

基金：

中国国家自然科学基金;

关键词：

capillary electrophoresis; tricarboxylic acid cycle; carboxylic acids; antibiotic;

D O I：

10.1016/j.jpba.2007.02.015

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Metabolomics is a new branch of systems biology exerting its influence in many aspects. In order to appraise the effects of antibiotics on central carbon metabolism, a CE based method was set up. With this platform, we estimated the organic acid metabolite pools' fluctuation of Escherichia coli and Pseudomonas aeruginosa cultured under 11 different antibiotics' stimuli. Multivariate data analysis showed that different antibiotics had clustered distributions for each strain and could be easily distinguished. Genetic, metabolic and antibiotic mechanism differences could also be deduced by the aid of further correlation analysis. For P. aeruginosa, even synergy action amid antibiotics could be ascertained. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：180 / 187

页数：8

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