On the mechanism of resistance to channel-forming colicins (PacB) and tellurite, encoded by plasmid Mip233 (IncHI3)

被引：14

作者：

Alonso, G ^{[1
]}

Gomes, C ^{[1
]}

González, C ^{[1
]}

Lemoine, VR ^{[1
]}

机构：

[1] Cent Univ Venezuela, Fac Ciencias, Inst Expt Biol, Caracas 1041A, Venezuela

来源：

FEMS MICROBIOLOGY LETTERS | 2000年 / 192卷 / 02期

关键词：

IncH plasmid; PacB; tellurite; CysK;

D O I：

10.1111/j.1574-6968.2000.tb09391.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Plasmids of the H incompatibility complex confer protection against all known channel-forming colicins (PacB character) and resistance to potassium tellurite (Te-r) to Escherichia coli strains. A DNA clone (2.2 kbp) from plasmid Mip233 (IncHI3) expressing PacB-Te-r phenotypes was studied. DNA sequence analysis revealed a high degree of homology with the enzyme O-acetylserine sulfhydrylase. Size of the PacB-Te-r transcript was estimated as 1200 bases. A single polypeptide was found on SDS-polyacrylamide gel with a molecular mass estimated of 34 kDa. The effect of channel-forming colicins and tellurite was analyzed at physiological and transcriptional levels. Results suggest that the pacB gene product could be a reductase-like enzyme. It is also suggested that presence of the PacB character among H plasmid confers selective advantage on cells sharing an ecological niche. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：257 / 261

页数：5

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