Solid-phase extraction with end-capped C2 columns for the routine measurement of racemic citalopram and metabolites in plasma by high-performance liquid chromatography

被引：40

作者：

Carlsson, B ^{[1
]}

Norlander, B ^{[1
]}

机构：

[1] Linkoping Univ, Fac Hlth Sci, Dept Clin Pharmacol, S-58183 Linkoping, Sweden

来源：

JOURNAL OF CHROMATOGRAPHY B | 1997年 / 702卷 / 1-2期

关键词：

citalopram; desmethylcitalopram; didesmethylcitalopram;

D O I：

10.1016/S0378-4347(97)00366-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An assay based on solid-phase extraction followed by high-performance liquid chromatography (HPLC) was developed for the measurement of citalopram and its main metabolites desmethylcitalopram and didesmethylcitalopram. The best extraction procedure was performed with end-capped C-2 column utilising secondary silanol interactions to obtain clean extract. The HPLC analysis was done on a phenyl column with a mobile phase without any amine additives. Fluorescence detection gave a limit of detection of 0.8 nmol/l plasma for the compounds analysed. (C) 1997 Elsevier Science B.V.

引用

页码：234 / 239

页数：6

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