Triplet state mechanism for electron transfer oxidation of DNA

被引：28

作者：

Ma, JH ^{[1
]}

Lin, WZ ^{[1
]}

Wang, WF ^{[1
]}

Han, ZH ^{[1
]}

Yao, SD ^{[1
]}

Lin, NY ^{[1
]}

机构：

[1] Chinese Acad Sci, Lab Radiat Chem, Shanghai Inst Nucl Res, Shanghai 201800, Peoples R China

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 2000年 / 57卷 / 01期

基金：

中国国家自然科学基金;

关键词：

DNA cleavage; DNA oxidation; anthraquinone-2-sulfonate;

D O I：

10.1016/S1011-1344(00)00081-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of anthraquinone-2-sulfonate with nucleotides and DNA in acetonitrile and acetonitrile water solvent mixture have been studied using KrF laser photolysis aimed at elucidation of the reaction mechanism. Laser spectroscopy directly demonstrates that the initial species from interaction of anthraquinone-2-sulfonate with nucleotides are radical cations of nucleotides and radical anion of anthraquinone-2-sulfonate. In addition, formation of ion pair from interaction of any of mucleotides with anthraquinone-2-sulfonate is synchronous with decay of triplet anthraquinone-2-sulfonate, which has provided dynamic evidence for initiation of electron transfer from DNA bases to tripler anthraquinone-2-sulfonate. Moreover, direct observation of stabilized DNA guanyl radical cation from interaction of anthraquinone-2-sulfonate with DNA has provided initial evidence for selective cleavage of DNA at guanine moiety. The solvent-separated ion pairs in acetonitrile have evidently dissociated into free ions, thereby enabling independent study of the behavior of guanyl radical cations and radical anion of anthraquinone-2-sulfonate. (C) 2000 Elsevier Science S.A. All rights reserved.

引用

页码：76 / 81

页数：6

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