Direct visualization of cholecystokinin subtype2 receptors in rat central nervous system using anti-peptide antibodies

被引：63

作者：

Mercer, LD ^{[1
]}

Le, VQ ^{[1
]}

Nunan, J ^{[1
]}

Jones, NM ^{[1
]}

Beart, PM ^{[1
]}

机构：

[1] Monash Univ, Dept Pharmacol, Clayton, Vic 3168, Australia

来源：

NEUROSCIENCE LETTERS | 2000年 / 293卷 / 03期

基金：

英国医学研究理事会;

关键词：

cholecystokinin2; receptor; antipeptide antibodies; immunohistochemistry; rat brain;

D O I：

10.1016/S0304-3940(00)01504-4

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The cholecystokinin receptor, subtype 2 (CCK2R), is considered, based on receptor autoradiography, to be the predominant receptor for this peptide transmitter in the mammalian central nervous system. To directly visualize the CCK2R we utilized a convenient and sensitive immunohistochemical procedure using antipeptide receptor antibodies raised in rabbits against unique portions of the carboxyl tail and third intracellular loop of the CCK2R. Antibodies were characterized by ELISA and Western blotting, and used for immunohistochemistry in rat brain sections. Studies with both antibodies revealed a widespread topographic distribution of CCK2R-like immunoreactivity (CCK2R-LI) in regions such as cortex, olfactory bulb, nucleus accumbens, septum, striatum, hippocampus, basolateral amygdala, habenula, hypothalamus, thalamus, ventral mesencephalon, inferior colliculus, parabrachial nucleus, pontine nucleus, supercolliculus, red nucleus, subcommisural and occulomotor nucleus, area postrema, solitary, olivary, cochlear, cuneate and trigeminal nuclei and spinal cord dorsal horn in agreement with the results of previous receptor autoradiography. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：167 / 170

页数：4

共 16 条

[1] IONIC MECHANISMS UNDERLYING CHOLECYSTOKININ ACTION IN RAT-BRAIN [J].

BODEN, P ;

WOODRUFF, GN .

CHOLECYSTOKININ, 1994, 713 :129-137

[2] AUTORADIOGRAPHIC LOCALIZATION OF CHOLECYSTOKININ A-RECEPTOR AND B-RECEPTOR IN RAT-BRAIN USING [I-125] D-TYR25 (NLE28,31)-CCK 25-33S [J].

CARLBERG, M ;

GUNDLACH, AL ;

MERCER, LD ;

BEART, PM .

EUROPEAN JOURNAL OF NEUROSCIENCE, 1992, 4 (06) :563-573

[3] Micromolar L-glutamate induces extensive apoptosis in an apoptotic-necrotic continuum of insult-dependent, excitotoxic injury in cultured cortical neurones [J].

Cheung, NS ;

Pascoe, CJ ;

Giardina, SF ;

John, CA ;

Beart, PM .

NEUROPHARMACOLOGY, 1998, 37 (10-11) :1419-1429

[4] BIOLOGICAL ACTIONS OF CHOLECYSTOKININ [J].

CRAWLEY, JN ;

CORWIN, RL .

PEPTIDES, 1994, 15 (04) :731-755

[5] Molecular cloning, developmental expression and pharmacological characterization of the CCKB gastrin receptor in the calf pancreas [J].

Dufresne, M ;

Escrieut, C ;

Clerc, P ;

LeHuerouLuron, I ;

Prats, H ;

Bertrand, V ;

LeMeuth, V ;

Guilloteau, P ;

Vaysse, N ;

Fourmy, D .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1996, 297 (1-2) :165-179

[6] DIFFERENTIATION OF CENTRAL CHOLECYSTOKININ RECEPTOR-BINDING SITES USING THE NONPEPTIDE ANTAGONISTS MK-329 AND L-365,260 [J].

HILL, DR ;

WOODRUFF, GN .

BRAIN RESEARCH, 1990, 526 (02) :276-283

[7] CHANGES IN HYPOTHALAMIC CHOLECYSTOKININ(A) AND CHOLECYSTOKININ(B) RECEPTOR SUBTYPES AND ASSOCIATED NEUROPEPTIDE EXPRESSION IN RESPONSE TO SALT-STRESS IN THE RAT AND MOUSE [J].

HINKS, GL ;

POAT, JA ;

HUGHES, J .

NEUROSCIENCE, 1995, 68 (03) :765-781

[8] DIFFERENTIAL GENE-EXPRESSION OF CCK(A) AND CCK(B) RECEPTORS IN THE RAT-BRAIN [J].

HONDA, T ;

WADA, E ;

BATTEY, JF ;

WANK, SA .

MOLECULAR AND CELLULAR NEUROSCIENCE, 1993, 4 (02) :143-154

[9]

JOHNSTONE A, 1984, IMMUNOCHEMISTRY PRAC

[10]

Mercer LD, 1999, NATO ADV SCI I A-LIF, V307, P175

← 1 2 →