Developmental regulation of protein phosphatase types 1 and 2A in post-hatch chicken brain

被引：12

作者：

Sim, ATR ^{[1
]}

Collins, E ^{[1
]}

Mudge, LM ^{[1
]}

Rostas, JAP ^{[1
]}

机构：

[1] Univ Newcastle, Fac Med & Hlth Sci, Discipline Med Biochem, Neurosci Grp, Callaghan, NSW 2308, Australia

来源：

NEUROCHEMICAL RESEARCH | 1998年 / 23卷 / 04期

基金：

英国医学研究理事会;

关键词：

protein phosphatase; protein phosphorylation; synaptogenesis; development; maturation;

D O I：

10.1023/A:1022422332404

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The activity and subcellular distribution of protein phosphatases I and 2A were measured in chicken forebrain and cerebellum during post-hatch development. At all post-hatch ages, a large proportion of PPI and PP2A was membrane bound and these enzymes were less active than their cytosolic counterparts. The protein concentration of PPI in the membranes increased 40% between 2 and 14 days and a further 60% between 14 days and adult, whereas the PPI enzyme activity in the membranes progressively decreased. In contrast to PP1, the protein concentration of PP2A remained constant in all fractions during post-hatch development, and the enzyme activity of PP2A did not change except for a decrease in the membrane-bound activity between 2 and 14 days. These results show that the subcellular distribution and activity of PP1 is selectively regulated during post-hatch development and that membrane association and inactivation of PP1 are independent events.

引用

页码：487 / 491

页数：5

共 22 条

[1]

ASZTALOS Z, 1993, J NEUROSCI, V13, P924

[2] PROTEIN-KINASE ACTIVITY CLOSELY ASSOCIATED WITH A RECONSTITUTED CALCIUM-ACTIVATED POTASSIUM CHANNEL [J].