Functional characterization of a Trypanosoma brucei TATA-binding protein-related factor points to a universal regulator of transcription in trypanosomes

被引:52
作者
Ruan, JP
Arhin, GK
Ullu, E
Tschudi, C
机构
[1] Yale Univ, Sch Med, Dept Epidemiol & Publ Hlth, New Haven, CT 06536 USA
[2] Yale Univ, Sch Med, Dept Internal Med, New Haven, CT 06536 USA
[3] Yale Univ, Sch Med, Dept Cell Biol, New Haven, CT 06536 USA
关键词
D O I
10.1128/MCB.24.21.9610-9618.2004
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transcriptional mechanisms remain poorly understood in trypanosomatid protozoa. In particular, there is no knowledge about the function of basal transcription factors, and there is an apparent rarity of promoters for protein-coding genes transcribed by RNA polymerase (Pol) II. Here we describe a Trypanosoma brucei factor related to the TATA-binding protein (TBP). Although this TBP-related factor (TBP-related factor 4 [TRF4]) has about 31% identity to the TBP core domain, several key residues involved in TATA box binding are not conserved. Depletion of the T. brucei TRF4 (TbTRF4) by RNA interference revealed an essential role in RNA Pol I, II, and III transcription. Using chromatin immunoprecipitation, we further showed that TRF4 is recruited to the Pol I-transcribed procyclic acidic repetitive genes, Poll II-transcribed spliced leader RNA genes, and Pol III-transcribed U-snRNA and 7SL RNA genes, thus supporting a role for TbTRF4 in transcription performed by all three nuclear RNA polymerases. Finally, a search for TRF4 binding sites in the T. brucei genome led to the identification of such sites in the 3' portion of certain protein-coding genes, indicating a unique aspect of Pol II transcription in these organisms.
引用
收藏
页码:9610 / 9618
页数:9
相关论文
共 41 条
[1]   A novel epitope tag system to study protein targeting and organelle biogenesis in Trypanosoma brucei [J].
Bastin, P ;
Bagherzadeh, A ;
Matthews, KR ;
Gull, K .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1996, 77 (02) :235-239
[2]   TBP-like factors come into focus [J].
Berk, AJ .
CELL, 2000, 103 (01) :5-8
[3]   Biochemistry and structural biology of transcription factor IID (TFIID) [J].
Burley, SK ;
Roeder, RG .
ANNUAL REVIEW OF BIOCHEMISTRY, 1996, 65 :769-799
[4]   Unbiased mapping of transcription factor binding sites along human chromosomes 21 and 22 points to widespread regulation of noncoding RNAs [J].
Cawley, S ;
Bekiranov, S ;
Ng, HH ;
Kapranov, P ;
Sekinger, EA ;
Kampa, D ;
Piccolboni, A ;
Sementchenko, V ;
Cheng, J ;
Williams, AJ ;
Wheeler, R ;
Wong, B ;
Drenkow, J ;
Yamanaka, M ;
Patel, S ;
Brubaker, S ;
Tammana, H ;
Helt, G ;
Struhl, K ;
Gingeras, TR .
CELL, 2004, 116 (04) :499-509
[5]   Life without transcriptional control? From fly to man and back again [J].
Clayton, CE .
EMBO JOURNAL, 2002, 21 (08) :1881-1888
[6]   TBP-like factor is required for embryonic RNA polymerase II transcription in C-elegans [J].
Dantonel, JC ;
Quintin, S ;
Lakatos, L ;
Labouesse, M ;
Tora, L .
MOLECULAR CELL, 2000, 6 (03) :715-722
[7]   The TBP-like factor: an alternative transcription factor in Metazoa? [J].
Dantonel, JC ;
Wurtz, JM ;
Poch, O ;
Moras, D ;
Tora, L .
TRENDS IN BIOCHEMICAL SCIENCES, 1999, 24 (09) :335-339
[8]   RNA polymerase II-dependent transcription in trypanosomes is associated with a SNAP complex-like transcription factor [J].
Das, A ;
Bellofatto, V .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (01) :80-85
[9]   The sequence and analysis of Trypanosoma brucei chromosome II [J].
El-Sayed, NMA ;
Ghedin, E ;
Song, JM ;
MacLeod, A ;
Bringaud, F ;
Larkin, C ;
Wanless, D ;
Peterson, J ;
Hou, LH ;
Taylor, S ;
Tweedie, A ;
Biteau, N ;
Khalak, HG ;
Lin, XY ;
Mason, T ;
Hannick, L ;
Caler, E ;
Blandin, G ;
Bartholomeu, D ;
Simpson, AJ ;
Kaul, S ;
Zhao, H ;
Pai, G ;
Van Aken, S ;
Utterback, T ;
Haas, B ;
Koo, HL ;
Umayam, L ;
Suh, B ;
Gerrard, C ;
Leech, V ;
Qi, R ;
Zhou, SG ;
Schwartz, D ;
Feldblyum, T ;
Salzberg, S ;
Tait, A ;
Turner, CMR ;
Ullu, E ;
White, O ;
Melville, S ;
Adams, MD ;
Fraser, CM ;
Donelson, JE .
NUCLEIC ACIDS RESEARCH, 2003, 31 (16) :4856-4863
[10]   RNA-POLYMERASE III-MEDIATED TRANSCRIPTION OF THE TRYPANOSOME U2 SMALL NUCLEAR-RNA GENE IS CONTROLLED BY BOTH INTRAGENIC AND EXTRAGENIC REGULATORY ELEMENTS [J].
FANTONI, A ;
DARE, AO ;
TSCHUDI, C .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (03) :2021-2028