Ataxin-2 interacts with the DEAD/H-box RNA helicase DDX6 and interferes with P-bodies and stress granules

被引：266

作者：

Nonhoff, Ute ^{[1
]}

Ralser, Markus ^{[1
]}

Welzel, Franziska ^{[1
]}

Piccini, Ilaria ^{[1
]}

Balzereit, Daniela ^{[1
]}

Yaspo, Marie-Laure ^{[1
]}

Lehrach, Hans ^{[1
]}

Krobitsch, Sylvia ^{[1
]}

机构：

[1] Max Planck Inst Mol Genet, D-14195 Berlin, Germany

来源：

MOLECULAR BIOLOGY OF THE CELL | 2007年 / 18卷 / 04期

关键词：

D O I：

10.1091/mbc.E06-12-1120

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Tight control of translation is fundamental for eukaryotic cells, and deregulation of proteins implicated contributes to numerous human diseases. The neurodegenerative disorder spinocerebellar ataxia type 2 is caused by a trinucleotide expansion in the SCA2 gene encoding a lengthened polyglutamine stretch in the gene product ataxin-2, which seems to be implicated in cellular RNA-processing pathways and translational regulation. Here, we substantiate a function of ataxin-2 in such pathways by demonstrating that ataxin-2 interacts with the DEAD/H-box RNA helicase DDX6, a component of P-bodies and stress granules, representing cellular structures of mRNA triage. We discovered that altered ataxin-2 levels interfere with the assembly of stress granules and cellular P-body structures. Moreover, ataxin-2 regulates the intracellular concentration of its interaction partner, the poly(A)-binding protein, another stress granule component and a key factor for translational control. Thus, our data imply that the cellular ataxin-2 concentration is important for the assembly of stress granules and P-bodies, which are main compartments for regulating and controlling mRNA degradation, stability, and translation.

引用

页码：1385 / 1396

页数：12

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