Mapping the interaction of bradykinin 1-5 with the exodomain of human protease activated receptor 4

被引：13

作者：

Nieman, MT

Pagan-Ramo, E

Warnock, M

Krijanovski, Y

Hasan, AAK

Schmaier, AH ^{[1
]}

机构：

[1] Univ Michigan, Dept Internal Med, Ann Arbor, MI 48109 USA

[2] Thromgen Inc, Ann Arbor, MI 48104 USA

来源：

FEBS LETTERS | 2005年 / 579卷 / 01期

关键词：

thrombin; PAR4; bradykinin; 1-5; RPPGF;

D O I：

10.1016/j.febslet.2004.11.041

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The angiotensin converting enzyme breakdown product of bradykinin, bradykinin 1-5 (RPPGF), inhibits thrombin-induced human or mouse platelet aggregation. RPPGF binds to the exodomain of human protease-activated receptor 1 (PAR1). Studies determined if RPPGF also binds to the exodomain of human PAR4. RPPGF binds to a peptide of the thrombin cleavage site on PAR4. Recombinant wild-type and mutated exodomain of human PAR4 was prepared. The N-terminal arginine on RPPGF binds to the P2 position or proline 46 on PAR4 to block thrombin cleavage. These data indicate that RPPGF influences thrombin activity by binding to the thrombin cleavage site on both PAR4 and PAR1 (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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页码：25 / 29

页数：5

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