Aluminum trichloride inhibits osteoblastic differentiation through inactivation of Wnt/β-catenin signaling pathway in rat osteoblasts

被引：21

作者：

Cao, Zheng ^{[1
]}

Fu, Yang ^{[1
]}

Sun, Xudong ^{[1
]}

Zhang, Qiuyue ^{[1
]}

Xu, Feibo ^{[1
]}

Li, Yanfei ^{[1
]}

机构：

[1] Northeast Agr Univ, Coll Vet Med, 59 Mucai St, Harbin 150030, Peoples R China

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2016年 / 42卷

基金：

中国国家自然科学基金;

关键词：

Aluminum trichloride; Osteoblastic differentiation; Wnt/beta-catenin pathway; Rat osteoblasts; BETA-CATENIN; PARATHYROID-HORMONE; BONE; MINERALIZATION; GROWTH; EXPRESSION; MECHANISM; RECEPTOR; BEHAVIOR; DISEASE;

D O I：

10.1016/j.etap.2015.11.023

中图分类号：

X [环境科学、安全科学];

学科分类号：

083001 [环境科学];

摘要：

Exposure to aluminum (Al) suppresses bone formation. Osteoblastic differentiation plays a key role in the process of bone formation. However, the effect of Al on osteoblastic differentiation is still controversial, and the mechanism remains unclear. To investigate the effect of Al on osteoblastic differentiation and whether Wnt signaling pathway was involved in it, the primary rat osteoblasts were exposed to 1/40 IC50, 1/20 IC50 and 1/10 IC50 of aluminum trichloride (AlCl3) for 24h, respectively. The activity analysis of alkaline phosphate, qRT-PCR analysis of type I collagen, alkaline phosphate, Wnt3a and Dkk-1, Western blot analysis of p-GSK3 beta, GSK3 beta and beta-catenin protein and Immunofluorescence staining for beta-catenin suggested that AlCl3 inhibited osteoblastic differentiation and Wnt/beta-catenin pathway. Moreover, we found exogenous Wnt3a application reversed the inhibitory effect of AlCl3 on osteoblastic differentiation, accompanied by activating the Wnt/beta-catenin pathway. Taken together, these findings suggest that AlCl3 inhibites osteoblastic differentiation through inactivation of Wnt/beta-catenin pathway in osteoblasts. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：198 / 204

页数：7

共 68 条

[1]

Acceleration of bone regeneration by local application of lithium: Wnt signal-mediated osteoblastogenesis and Wnt signal-independent suppression of osteoclastogenesis [J].