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Impact of aging on rat bone marrow-derived stem cell chondrogenesis

被引：77
作者：
Zheng, Hongjun
Martin, James A.
Duwayri, Yazan
Falcon, Gilbert
Buckwalter, Joseph A.
机构：
[1] Univ Iowa, Coll Med, Dept Orthoped, Iowa City, IA 52242 USA
[2] Univ Iowa, Coll Med, Dept Orthopaed & Rehabil, Iowa City, IA 52242 USA
[3] Med Univ Ohio, Dept Surg, Toledo, OH USA
[4] N Dakota Coll Med & Hlth Sci, Grand Forks, ND USA
来源：
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES | 2007年 / 62卷 / 02期
关键词：
TIBIAL PLAFOND FRACTURES; DEGENERATIVE ARTHRITIS; DIFFERENTIATION; CARTILAGE; AGE; REDUCTION; RABBIT; REPAIR; SUBPOPULATION; SENESCENCE;
D O I：
10.1093/gerona/62.2.136
中图分类号：
R592 [老年病学]; C [社会科学总论];
学科分类号：
03 ; 0303 ; 100203 ;
摘要：
Damaged articular cartilage rarely heals or regenerates in middle-aged and elderly adults, suggesting that the chondrogenic potential of mesenchymal stem cells declines with age. To test this hypothesis, we measured the responses of rat bone marrow-derived mesenchymal stem cells (BMSCs) to chondrogenic induction in vitro. BMSCs from immature rats (1 week old), young adult rats (12 weeks old), and old adult rats (1 year old) were analyzed for cartilage extracellular matrix (ECM) production. Histologic analysis showed strong cartilage ECM formation by BMSCs from 1-week-old rats, but not by BMSCs from 12-week-old or 1-year-old rats. Real-time polymerase chain reaction revealed age-related declines in messenger RNA encoding type II collagen, aggrecan, and link protein, three major cartilage ECM components. Microarray analysis indicated significant age-related differences in the expression of genes that influence cartilage ECM formation. These findings support the hypothesis that the chondrogenic potential of mesenchymal stem cells declines with age.
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页码：136 / 148
页数：13
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