Hepatocyte growth factor promotes neuronal differentiation of neural stem cells derived from embryonic stem cells

被引：23

作者：

Kato, M

Yoshimura, S

Kokuzawa, J

Kitajima, H

Kaku, Y

Warria, T

Shinoda, J

Takahiro, KI

Sakai, N

机构：

[1] Gifu Univ, Sch Med, Dept Neurosurg, Div Neurosci, Gifu 5008705, Japan

[2] Gifu Univ, Sch Med, Dept Tissue & Organ Dev Regenerat & Adv Med Sci, Gifu 5008705, Japan

来源：

NEUROREPORT | 2004年 / 15卷 / 01期

关键词：

differentiation; embryonic stem cell; hepatocyte growth factor; neural stem cells;

D O I：

10.1097/00001756-200401190-00002

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We previously reported that hepatocyte growth factor (HGF) promoted proliferation of neurospheres and neuronal differentiation of neural stem cells (NSCs) derived from mouse embryonic brain. In this study, spheres from mouse embryonic stem (ES) cells were generated by floating culture following co-culture on PA6 stromal cells. In contrast to the behavior of the neurospheres derived from embryonic brain, addition of HGF to the growth medium of the floating cultures decreased the number of spheres derived from ES cells. When spheres were stained using a MAP-2 antibody, more MAP-2-positive cells were observed in spheres cultured with HGF. When HGF was added to the growth and/or differentiation medium, more MAP-2-positive cells were also obtained. These results suggest that HGF promotes neuronal differentiation of NSCs derived from ES cells.

引用

页码：5 / 8

页数：4

共 18 条

[1] Insulin-like growth factor-1 is necessary for neural stem cell proliferation and demonstrates distinct actions of epidermal growth factor and fibroblast growth factor-2 [J].