Extracellular purines from cells of seminiferous tubules

被引:28
作者
Gelain, DP [1 ]
de Souza, LF [1 ]
Bernard, EA [1 ]
机构
[1] Univ Fed Rio Grande do Sul, ICBS, Dept Bioquim, Lab Transducao Sinal Celulas Testiculares, BR-90035003 Porto Alegre, RS, Brazil
关键词
Sertoli; extracellular nucleotides; germ cells; peritubular cells; seminiferous tubules; purinoceptors;
D O I
10.1023/A:1022857608849
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
It has been long postulated that extracellular purines can modulate the function of the male reproductive system by interacting with different purinergic receptors of Sertoli and germinative cells. Many authors have described the biological changes induced by extracellular ATP and/or adenosine in these cells, and some hypothetical models for paracrine communication mediated by purines were proposed; however, the cellular source(s) of these molecules in seminiferous tubules remains unknown. In this study, we demonstrated for the first time that Sertoli cells are able to release ATP (0.3 nmol/mg protein) and adenosine (0.1 nmol/mg protein) in the extracellular medium, while germinative and myoid peritubular cells are able to secrete adenosine (0.02 and 0.37 nmol/mg protein, respectively). Indeed, all the three types of cells were able to release inosine at significant concentrations (about 0.4 nmol/mg protein). This differential secretion depending on the cellular type suggests that these molecules may be involved in the paracrine regulation and/or control of the maturation processes of these cells.
引用
收藏
页码:1 / 9
页数:9
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