Candidate reference genes for gene expression studies in water lily

被引：44

作者：

Luo, Huolin ^{[1
]}

Chen, Sumei ^{[1
]}

Wan, Hongjian ^{[1
]}

Chen, Fadi ^{[1
]}

Gu, Chunsun ^{[1
]}

Liu, Zhaolei ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2010年 / 404卷 / 01期

关键词：

REAL-TIME PCR; POLYMERASE-CHAIN-REACTION; RT-PCR; INTERNAL CONTROL; SELECTION; QUANTIFICATION; NORMALIZATION; MODEL;

D O I：

10.1016/j.ab.2010.05.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The selection of an appropriate reference gene(s) is a prerequisite for the proper interpretation of quantitative Real-Time polymerase chain reaction data. We report the evaluation of eight candidate reference genes across various tissues and treatments in the water lily by the two software packages geNorm and NormFinder. Across all samples, clathrin adaptor complexes medium subunit (AP47) and actin 11 (ACT11) emerged as the most suitable reference genes. Across different tissues, ACT11 and elongation factor 1-alpha (EF1 alpha) exhibited a stable expression pattern. ACTH and AP47 also stably expressed in roots subjected to various treatments, but in the leaves of the same plants the most stably expressed genes were ubiquitin-conjugating enzyme 16 (UBC16) and ACT11. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：100 / 102

页数：3

共 13 条

[1] Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets [J].

Andersen, CL ;

Jensen, JL ;

Orntoft, TF .

CANCER RESEARCH, 2004, 64 (15) :5245-5250

[2] Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems [J].

Bustin, SA .

JOURNAL OF MOLECULAR ENDOCRINOLOGY, 2002, 29 (01) :23-39

[3] Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process [J].

Exposito-Rodriguez, Marino ;

Borges, Andres A. ;

Borges-Perez, Andres ;

Perez, Jose A. .

BMC PLANT BIOLOGY, 2008, 8 (1)

[4] Gene quantification using real-time quantitative PCR: An emerging technology hits the mainstream [J].

Ginzinger, DG .

EXPERIMENTAL HEMATOLOGY, 2002, 30 (06) :503-512

[5] Exploring valid reference genes for gene expression studies in Brachypodium distachyon by real-time PCR [J].

Hong, Shin-Young ;

Seo, Pil Joon ;

Yang, Moon-Sik ;

Xiang, Fengning ;

Park, Chung-Mo .

BMC PLANT BIOLOGY, 2008, 8 (1)

[6] Evaluation of putative reference genes for gene expression normalization in soybean by quantitative real-time RT-PCR [J].

Hu, Ruibo ;

Fan, Chengming ;

Li, Hongyu ;

Zhang, Qingzhu ;

Fu, Yong-Fu .

BMC MOLECULAR BIOLOGY, 2009, 10 :93

[7] Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR [J].

Jain, Mukesh ;

Nijhawan, Aashima ;

Tyagi, Akhilesh K. ;

Khurana, Jitendra P. .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 345 (02) :646-651

[8] Selection of reference genes for quantitative real-time PCR in a rat asphyxial cardiac arrest model [J].

Langnaese, Kristina ;

John, Robin ;

Schweizer, Hannes ;

Ebmeyer, Uwe ;

Keilhoff, Gerburg .

BMC MOLECULAR BIOLOGY, 2008, 9

[9] Reference gene selection for quantitative real-time PCR normalization in tomato subjected to nitrogen, cold, and light stress [J].

Lovdal, Trond ;

Lillo, Cathrine .

ANALYTICAL BIOCHEMISTRY, 2009, 387 (02) :238-242

[10] Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data [J].

Ramakers, C ;

Ruijter, JM ;

Deprez, RHL ;

Moorman, AFM .

NEUROSCIENCE LETTERS, 2003, 339 (01) :62-66

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