Genotyping accuracy for whole-genome amplification of DNA from buccal epithelial cells

被引：6

作者：

Leviel, K ^{[1
]}

Olarte, M ^{[1
]}

Sullivan, PF ^{[1
]}

机构：

[1] Univ N Carolina, Dept Genet, Chapel Hill, NC 27599 USA

来源：

TWIN RESEARCH | 2004年 / 7卷 / 05期

关键词：

D O I：

10.1375/twin.7.5.482

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We compared the accuracy of genotyping for DNA extracted from lymphocytes to that of DNA amplified from buccal epithelial cells. Amplification was via a rolling circle/phi29 DNA polymerase commercial kit. Paired buccal and lymphocyte DNA samples were available from 30 individuals. All samples were genotyped for 12 SNPs, 5 microsatellites and 2 VNTRs. The accuracy of genotyping (no-call proportions, reproducibility, and concordance) was similar for DNA from lymphocytes in comparison to amplified DNA from buccal samples. If used with caution, these data suggest that rolling-circle whole-genome amplification can be used to increase the DNA mass available for large-scale genotyping projects based on DNA from buccal cells.

引用

页码：482 / 484

页数：3

共 12 条

[1]

[Anonymous], BIOTECHNIQUES S

[2] Two methods of whole-genome amplification enable accurate genotyping across a 2320-SNP linkage panel [J].

Barker, DL ;

Hansen, MST ;

Faruqi, AF ;

Giannola, D ;

Irsula, OR ;

Lasken, RS ;

Latterich, M ;

Makarov, V ;

Oliphant, A ;

Pinter, JH ;

Shen, R ;

Sleptsova, I ;

Ziehler, W ;

Lai, E .

GENOME RESEARCH, 2004, 14 (05) :901-907

[3] Comprehensive human genome amplification using multiple displacement amplification [J].

Dean, FB ;

Hosono, S ;

Fang, LH ;

Wu, XH ;

Faruqi, AF ;

Bray-Ward, P ;

Sun, ZY ;

Zong, QL ;

Du, YF ;

Du, J ;

Driscoll, M ;

Song, WM ;

Kingsmore, SF ;

Egholm, M ;

Lasken, RS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (08) :5261-5266

[4] Unbiased whole-genome amplification directly from clinical samples [J].

Hosono, S ;

Faruqi, AF ;

Dean, FB ;

Du, YF ;

Sun, ZY ;

Wu, XH ;

Du, J ;

Kingsmore, SF ;

Egholm, M ;

Lasken, RS .

GENOME RESEARCH, 2003, 13 (05) :954-964

[5]

Kang Ji C., 1999, Mycoscience, V40, P151, DOI 10.1007/BF02464294

[6] Association of anxiety-related traits with a polymorphism in the serotonin transporter gene regulatory region [J].

Lesch, KP ;

Bengel, D ;

Heils, A ;

Sabol, SZ ;

Greenberg, BD ;

Petri, S ;

Benjamin, J ;

Muller, CR ;

Hamer, DH ;

Murphy, DL .

SCIENCE, 1996, 274 (5292) :1527-1531

[7]

Nelson JR, 2002, BIOTECHNIQUES, P44

[8] Genome coverage and sequence fidelity of φ29 polymerase-based multiple strand displacement whole genome amplification -: art. no. e71 [J].

Paez, JG ;

Lin, M ;

Beroukhim, R ;

Lee, JC ;

Zhao, XJ ;

Richter, DJ ;

Gabriel, S ;

Herman, P ;

Sasaki, H ;

Altshuler, D ;

Li, C ;

Meyerson, M ;

Sellers, WR .

NUCLEIC ACIDS RESEARCH, 2004, 32 (09) :e71

[9]

Roberts R, 2000, HUM MUTAT, V16, P77, DOI 10.1002/1098-1004(200007)16:1<77::AID-HUMU13>3.3.CO

[10]

2-K

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