Crystallization and preliminary crystallographic studies of mung bean cytokinin-specific binding protein

被引：13

作者：

Bujacz, GD

Pasternak, O

Fujimoto, Y

Hashimoto, Y

Sikorski, MM

Jaskolski, M ^{[1
]}

机构：

[1] Polish Acad Sci, Inst Bioorgan Chem, Poznan, Poland

[2] Tech Univ Lodz, Inst Tech Biochem, PL-90924 Lodz, Poland

[3] Teikyo Univ, Fac Pharmaceut Sci, Tokyo 173, Japan

[4] Univ Tokyo, Inst Mol & Cellular Biosci, Tokyo, Japan

[5] Adam Mickiewicz Univ Poznan, Fac Chem, Dept Crystallog, Poznan, Poland

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2003年 / 59卷

关键词：

D O I：

10.1107/S0907444902022916

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytokinins, or plant growth hormones, bind with very high affinity to cytokinin-specific binding proteins (CSBPs). Recombinant mung bean CSBP has been overexpressed in Escherichia coli and crystallized in complex with zeatin, a natural plant growth hormone. The crystals belong to the hexagonal system, space group P6(2) or P6(4), with unit-cell parameters a = 113.62, c = 86.85 Angstrom, contain two to five copies of the protein in the asymmetric unit and diffract X-rays to 1.25 Angstrom resolution.

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页码：522 / 525

页数：4

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