Determination of metalloproteinases, plasminogen-activators and their inhibitors in the synovial fluids of patients with rheumatoid arthritis during chemical synoviorthesis

被引:48
作者
Blaser, J
Triebel, S
Maasjosthusmann, U
Romisch, J
KrahlMateblowski, U
Freudenberg, W
Fricke, R
Tschesche, H
机构
[1] UNIV BIELEFELD,INST BIOCHEM,D-33615 BIELEFELD,GERMANY
[2] ST JOSEFS STIFT SENDENHORST,KLIN RHEUMATOL,BIELEFELD,GERMANY
[3] BEHRINGWERKE AG,RES LABS,W-3550 MARBURG,GERMANY
关键词
metalloproteinases; urokinase-plasminogen-activator; rheumatoid arthritis; synoviorthesis;
D O I
10.1016/0009-8981(95)06172-X
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
The concentrations of matrix metalloproteinase-l (MMP-1), matrix metalloproteinase-8 (MMP-8), matrix metalloproteinase-9 (MMP-8), lactoferrin and urokinase plasminogen activator (uPA), tissue-type plasminogen activator (tPA) and the inhibitors, tissue inhibitor of metalloproteinase-l (TIMP-1), plasminogen activator inhibitor-1 (PAI-1), plasminogen activator inhibitor (PAI-2), and alpha(2)-macroglobulin in the synovial fluids of patients with rheumatoid arthritis was determined before and during chemical synoviorthesis with a sodium salt of the fatty acids from cod-liver oil (Varicocid). synovial fluids were obtained before treatment from 37 patients with rheumatoid arthritis and, in most cases, at 8 and 24 h after injection of the agent. Well-established ELISAs were used to determine the amounts of all proteins. All patients with rheumatoid arthritis revealed very high levels of metalloproteinases (about 1-15 mu g/ml) in their synovial fluids. During the inflammation inducing treatment the granulocyte enzymes increased. In contrast to this, the level of MMP-1 decreased. All granulocyte-derived enzymes were strongly correlated with each other, whereas their dependence on the granulocyte count was only weak. uPA and PAI-2 showed good correlations with the granulocytes-derived enzymes, but were also only weakly correlating with the cell counts. tPA was not detected by the ELISA used. The proteases, MMP-8, MMP9 and uPA were increased 8 h after the treatment, whereas the specific inhibitors TIMP-1, PAI-1 and PAI-2 showed significant changes only 24 h after the injection. Matrix metalloproteinases are important factors in the pathogenesis of rheumatoid arthritis. The inflammatory activity in the joint could be better correlated to the granulocyte enzymes than to the granulocyte counts. The levels of uPA and PA1-2 are also parallel to the granulocyte enzyme levels and might underly the same regulatory mechanism.
引用
收藏
页码:17 / 33
页数:17
相关论文
共 37 条
[1]   PROTEOLYTIC INACTIVATION OF ALPHA-1-ANTITRYPSIN AND ALPHA-1-ANTICHYMOTRYPSIN BY NEUTROPHILS IN ARTHRITIC JOINTS [J].
ABBINK, JJ ;
KAMP, AM ;
NUIJENS, JH ;
SWAAK, TJG ;
HACK, CE .
ARTHRITIS AND RHEUMATISM, 1993, 36 (02) :168-180
[2]  
ARMOUR PC, 1984, RHEUMATOL INT, V4, P151, DOI 10.1007/BF00541205
[3]   THE AMERICAN-RHEUMATISM-ASSOCIATION 1987 REVISED CRITERIA FOR THE CLASSIFICATION OF RHEUMATOID-ARTHRITIS [J].
ARNETT, FC ;
EDWORTHY, SM ;
BLOCH, DA ;
MCSHANE, DJ ;
FRIES, JF ;
COOPER, NS ;
HEALEY, LA ;
KAPLAN, SR ;
LIANG, MH ;
LUTHRA, HS ;
MEDSGER, TA ;
MITCHELL, DM ;
NEUSTADT, DH ;
PINALS, RS ;
SCHALLER, JG ;
SHARP, JT ;
WILDER, RL ;
HUNDER, GG .
ARTHRITIS AND RHEUMATISM, 1988, 31 (03) :315-324
[4]  
BERGMANN U, 1989, J CLIN CHEM CLIN BIO, V27, P351
[5]   MATRIX METALLOPROTEINASES - A REVIEW [J].
BIRKEDALHANSEN, H ;
MOORE, WGI ;
BODDEN, MK ;
WINDSOR, LJ ;
BIRKEDALHANSEN, B ;
DECARLO, A ;
ENGLER, JA .
CRITICAL REVIEWS IN ORAL BIOLOGY & MEDICINE, 1993, 4 (02) :197-250
[6]   DETECTION OF INTERLEUKIN-8 BIOLOGICAL-ACTIVITY IN SYNOVIAL-FLUIDS FROM PATIENTS WITH RHEUMATOID-ARTHRITIS AND PRODUCTION OF INTERLEUKIN-8 MESSENGER-RNA BY ISOLATED SYNOVIAL-CELLS [J].
BRENNAN, FM ;
ZACHARIAE, COC ;
CHANTRY, D ;
LARSEN, CG ;
TURNER, M ;
MAINI, RN ;
MATSUSHIMA, K ;
FELDMANN, M .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1990, 20 (09) :2141-2144
[7]   LYSIS OF FIBRILLAR COLLAGEN BY NEUTROPHILS IN SYNOVIAL-FLUID - A ROLE FOR SURFACE-BOUND IMMUNOGLOBULINS [J].
CHATHAM, WW ;
HECK, LW ;
BLACKBURN, WD .
ARTHRITIS AND RHEUMATISM, 1990, 33 (09) :1333-1339
[8]  
CHATHAM WW, 1993, ARTHRITIS RHEUM, V36, P51
[9]   THE MEASUREMENT OF COLLAGENASE, TISSUE INHIBITOR OF METALLOPROTEINASES (TIMP), AND COLLAGENASE TIMP COMPLEX IN SYNOVIAL-FLUIDS FROM PATIENTS WITH OSTEOARTHRITIS AND RHEUMATOID-ARTHRITIS [J].
CLARK, IM ;
POWELL, LK ;
RAMSEY, S ;
HAZLEMAN, BL ;
CAWSTON, TE .
ARTHRITIS AND RHEUMATISM, 1993, 36 (03) :372-379
[10]   INSITU HYBRIDIZATION STUDIES OF STROMELYSIN AND COLLAGENASE MESSENGER-RNA EXPRESSION IN RHEUMATOID SYNOVIUM [J].
GRAVALLESE, EM ;
DARLING, JM ;
LADD, AL ;
KATZ, JN ;
GLIMCHER, LH .
ARTHRITIS AND RHEUMATISM, 1991, 34 (09) :1076-1084