The separation of biomolecules using capillary electrochromatography

被引：41

作者：

Fu, HJ ^{[1
]}

Huang, XD ^{[1
]}

Jin, WH ^{[1
]}

Zou, HF ^{[1
]}

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2003年 / 14卷 / 01期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1016/S0958-1669(02)00006-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The unique properties of capillary electrochromatography such as high performance, high selectivity, minimum consumption of both reagents and samples, and good compatibility with mass spectrometry make this technique an attractive one for the analysis of biomolecules including peptides, proteins, carbohydrates, nucleosides and nucleoticles. Irreversible adsorption between the biomolecules and the charged packing surface leads to a lack of reproducibility and serious peak tailing, so various approaches have been taken to overcome this and to improve the technique for future challenges.

引用

页码：96 / 100

页数：5

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