Monitoring of specific methanogenic activity of granular sludge by confocal laser scanning microscopy during start-up of thermophilic upflow anaerobic sludge blanket reactor

被引：20

作者：

Ahn, Y

Lee, YJ

Kim, HS

Park, S ^{[1
]}

机构：

[1] Pusan Natl Univ, Dept Chem Engn, Pusan 609735, South Korea

[2] Pusan Natl Univ, Inst Environm Technol & Ind, Pusan 609735, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 20期

基金：

新加坡国家研究基金会;

关键词：

coenzyme F; confocal microscopy; fluorescence; granular sludge; methanogen;

D O I：

10.1023/A:1005696017394

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Confocal, laser-scanning microscopy was applied to acquire coenzyme F-420-based autofluorescence images of middle sections of sludge granules during start-up of a thermophilic reactor that were seeded with mesophilically-grown microorganisms of granular sludge. Digital images were analyzed to calculate weighted averages of autofluorescence. The values were related (r(2)=0.97) to specific methanogenic activities of granular sludge as the granules developed to steady state.

引用

页码：1591 / 1596

页数：6

共 19 条

[1] Isolation and characterization of methanophenazine and function of phenazines in membrane-bound electron transport of Methanosarcina mazei Gol [J].