Amphipathic helix-dependent localization of NS5A mediates hepatitis C virus RNA replication

被引：142

作者：

Elazar, M

Cheong, KH

Liu, P

Greenberg, HB

Rice, CM

Glenn, JS

机构：

[1] Stanford Univ, Sch Med, Div Gastroenterol & Hepatol, Stanford, CA 94305 USA

[2] Vet Adm Med Ctr, Dept Microbiol & Immunol, Palo Alto, CA 94304 USA

[3] Rockefeller Univ, Ctr Study Hepatitis C, New York, NY 10021 USA

来源：

JOURNAL OF VIROLOGY | 2003年 / 77卷 / 10期

关键词：

D O I：

10.1128/JVI.77.10.6055-6061.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We identified an N-terminal amphipathic helix (AH) in one of hepatitis C virus (HCV)'s nonstructural proteins, NS5A. This AH is necessary and sufficient for membrane localization and is conserved across isolates. Genetically disrupting the AH impairs HCV replication. Moreover, an AH peptide-mimic inhibits the membrane association of NS5A in a dose-dependent manner. These results have exciting implications for the HCV life cycle and novel antiviral strategies.

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页码：6055 / 6061

页数：7

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