Hyperphagocytic haemocytes in Manduca sexta

被引:33
作者
Dean, P
Potter, U
Richards, EH
Edwards, JP
Charnley, AK
Reynolds, SE
机构
[1] Univ Bristol, Dept Pathol & Microbiol, Bristol BS8 1TD, Avon, England
[2] Univ Bath, Dept Biol & Biochem, Bath BA2 7AY, Avon, England
[3] Univ Bath, Ctr Electron Opt Studies, Bath BA2 7AY, Avon, England
[4] Cent Sci Lab, York YO14 1LZ, N Yorkshire, England
关键词
haemocytes; insect immunity; nodule formation; phagocytosis;
D O I
10.1016/j.jinsphys.2004.09.003
中图分类号
Q96 [昆虫学];
学科分类号
摘要
We have discovered a new type of haemocyte in the larval stage of the tobacco hornworm moth Manduca sexta that has extreme phagocytic ability; each cell can engulf up to 500 bacteria. This level of phagocytosis may be unprecedented among animal cells. Although these hyperphagocytic cells (HP) only represent about 1% of the circulating haemocytes, they are responsible for sequestering the majority of the bacteria by circulating haemocytes when non-pathogenic, heat-killed Escherichia coli are injected into the haemolymph. Extreme phagocytosis by HP is not limited to Gram-negative bacteria since heat-killed Staphylococcus aureus as well as positively and negatively charged microspheres are also highly phagocytosed. Evidence is presented to show that phagocytosis by HP is involved in the early stages of nodule formation in infected insects. In addition, HP are also present in noninfected insects, characterised by their distinctive spreading morphology, which becomes impaired following hyperphagocytosis of bacteria. This is the first time that a dedicated "professional" phagocytic class of haemocyte has been reported for an invertebrate. The importance of these specialised cell types in the M. sexta immune response and their role in nodule formation is discussed. (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1027 / 1036
页数:10
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