Real-time PCR-based method for assaying the purity of bacterial artificial chromosome preparations

被引：6

作者：

Foreman, PK

Davis, RW

机构：

[1] Genencor Int, Palo Alto, CA 94305 USA

[2] Stanford Univ, Sch Med, Stanford, CA 94305 USA

来源：

BIOTECHNIQUES | 2000年 / 29卷 / 03期

关键词：

D O I：

10.2144/00293bm01

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：410 / 412

页数：3

共 14 条

[1] Fluorescence-based sequencing directly from bacterial and P1-derived artificial chromosomes [J].

Boysen, C ;

Simon, MI ;

Hood, L .

BIOTECHNIQUES, 1997, 23 (06) :978-&

[2] Preparative separation of plasmid and bacterial artificial chromosome DNA by density gradient electrophoresis in the presence of linear polymers [J].

Cole, KD .

ELECTROPHORESIS, 1998, 19 (18) :3062-3068

[3] A modular, positive selection bacterial artificial chromosome vector with multiple cloning sites [J].

Frengen, E ;

Weichenhan, D ;

Zhao, BH ;

Osoegawa, K ;

van Geel, M ;

de Jong, PJ .

GENOMICS, 1999, 58 (03) :250-253

[4] SIMULTANEOUS AMPLIFICATION AND DETECTION OF SPECIFIC DNA-SEQUENCES [J].

HIGUCHI, R ;

DOLLINGER, G ;

WALSH, PS ;

GRIFFITH, R .

BIO-TECHNOLOGY, 1992, 10 (04) :413-417

[5] KINETIC PCR ANALYSIS - REAL-TIME MONITORING OF DNA AMPLIFICATION REACTIONS [J].

HIGUCHI, R ;

FOCKLER, C ;

DOLLINGER, G ;

WATSON, R .

BIO-TECHNOLOGY, 1993, 11 (09) :1026-1030

[6] DETECTION OF SPECIFIC POLYMERASE CHAIN-REACTION PRODUCT BY UTILIZING THE 5'-]3' EXONUCLEASE ACTIVITY OF THERMUS-AQUATICUS DNA-POLYMERASE [J].

HOLLAND, PM ;

ABRAMSON, RD ;

WATSON, R ;

GELFAND, DH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (16) :7276-7280

[7]

Kirschner LS, 1999, BIOTECHNIQUES, V27, P72

[8]

LIVAK KJ, 1995, PCR METH APPL, V4, P357

[9]

MOATMAN R, 1996, QIAGEN NEWS, V4

[10]

Nasarabadi S, 1999, BIOTECHNIQUES, V27, P1116

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