Mucosal immunization with a genetically engineered pertussis toxin S1 fragment-cholera toxin subunit B chimeric protein

被引：29

作者：

Lee, SF ^{[1
]}

Halperin, SA

Salloum, DF

MacMillan, A

Morris, A

机构：

[1] Dalhousie Univ, Fac Dent, Dept Appl Oral Sci, Halifax, NS B3H 3J5, Canada

[2] Dalhousie Univ, Dept Microbiol & Immunol, Halifax, NS B3H 3J5, Canada

[3] IWK Hlth Ctr, Dept Pediat, Halifax, NS B3H 3J5, Canada

来源：

INFECTION AND IMMUNITY | 2003年 / 71卷 / 04期

关键词：

D O I：

10.1128/IAI.71.4.2272-2275.2003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A chimeric protein consisting of a divalent pertussis toxin (PT) S1 fragment linked to the cholera toxin (Ctx) A(2)B fragment was constructed. The chimera induced a mucosal immunoglobulin A (IgA) and a serum IgG immune response to PT and CtxB in BALB/c mice following intranasal immunization. The immune sera neutralized PT in vitro. In the mouse model of Bordetella pertussis respiratory infection, the chimera-immunized animals showed a significant reduction in bacterial lung counts (P = 0.01) from that of the sham control group. Thus, a divalent S1 fragment CtxA2B chimera is an immunogenic antigen and can elicit a protective immunity.

引用

页码：2272 / 2275

页数：4

共 18 条

[1] CONSTRUCTION OF A DIPHTHERIA TOXIN-A FRAGMENT-C180 PEPTIDE FUSION PROTEIN WHICH ELICITS A NEUTRALIZING ANTIBODY-RESPONSE AGAINST DIPHTHERIA-TOXIN AND PERTUSSIS TOXIN [J].

BARBIERI, JT ;

ARMELLINI, D ;

MOLKENTIN, J ;

RAPPUOLI, R .

INFECTION AND IMMUNITY, 1992, 60 (12) :5071-5077

[2] NEUTRALIZING ANTIBODIES AND IMMUNOPROTECTION AGAINST PERTUSSIS AND TETANUS OBTAINED BY USE OF A RECOMBINANT PERTUSSIS TOXIN TETANUS TOXIN FUSION PROTEIN [J].

BOUCHER, P ;

SATO, H ;

SATO, Y ;

LOCHT, C .

INFECTION AND IMMUNITY, 1994, 62 (02) :449-456

[3] Protection against Bordetella pertussis infection following parenteral or oral immunization with antigens entrapped in biodegradable particles:: effect of formulation and route of immunization on induction of Th1 and Th2 cells [J].

Conway, MA ;

Madrigal-Estebas, L ;

McClean, S ;

Brayden, DJ ;

Mills, KHG .

VACCINE, 2001, 19 (15-16) :1940-1950

[4] HUMAN T-CELL CLONES DEFINE S1 SUBUNIT AS THE MOST IMMUNOGENIC MOIETY OF PERTUSSIS TOXIN AND DETERMINE ITS EPITOPE MAP [J].

DEMAGISTRIS, MT ;

ROMANO, M ;

BARTOLONI, A ;

RAPPUOLI, R ;

TAGLIABUE, A .

JOURNAL OF EXPERIMENTAL MEDICINE, 1989, 169 (05) :1519-1532

[5]

GILL DM, 1976, BIOCHEMISTRY-US, V12, P3558

[6]

HAJISHENGALLIS G, 1995, J IMMUNOL, V154, P4322

[7] MODULATION OF BORDETELLA-PERTUSSIS INFECTION WITH MONOCLONAL-ANTIBODIES TO PERTUSSIS TOXIN [J].

HALPERIN, SA ;

ISSEKUTZ, TB ;

KASINA, A .

JOURNAL OF INFECTIOUS DISEASES, 1991, 163 (02) :355-361

[8] Role of the C terminus in antigen P1 surface localization in Streptococcus mutans and two related cocci [J].

HomonyloMcGavin, MK ;

Lee, SF .

JOURNAL OF BACTERIOLOGY, 1996, 178 (03) :801-807

[9] Surface expression of a protective recombinant pertussis toxin S1 subunit fragment in Streptococcus gordonii [J].

Lee, SF ;

March, RJ ;

Halperin, SA ;

Faulkner, G ;

Gao, LQ .

INFECTION AND IMMUNITY, 1999, 67 (03) :1511-1516

[10] Oral colonization and immune responses to Streptococcus gordonii expressing a pertussis toxin S1 fragment in mice [J].

Lee, SF ;

Halperin, SA ;

Wang, H ;

MacArthur, A .

FEMS MICROBIOLOGY LETTERS, 2002, 208 (02) :175-178

← 1 2 →